BackgroundThe gastrointestinal (GI) mucosal cells turnover regularly under physiological conditions, which may be stimulated in various pathological situations including inflammation. Local epithelial stem cells appear to play a major role in such mucosal renewal or pathological regeneration. Less is clear about the involvement of multipotent stem cells from blood in GI repair. We attempted to explore a role of bone marrow mesenchymal stromal cells (BMMSCs) and soluble stem cell factor (SCF) in GI mucosa regeneration in a rat model of inflammatory bowel diseases (IBD).MethodsBMMSCs labelled with the fluorescent dye PKH26 from donor rats were transfused into rats suffering indomethacin-induced GI injury. Experimental effects by BMMSCs transplant and SCF were determined by morphometry of intestinal mucosa, double labeling of PKH26 positive BMMSCs with endogenous proliferative and intestinal cell markers, and western blot and PCR analyses of the above molecular markers in the recipient rats relative to controls.ResultsPKH26 positive BMMSCs were found in the recipient mucosa, partially colocalizing with the proliferating cell nuclear antigen (PCNA), Lgr5, Musashi-1 and ephrin-B3. mRNA and protein levels of PCNA, Lgr5, Musashi-1 and ephrin-B3 were elevated in the intestine in BMMSCs-treated rats, most prominent in the BMMSCs-SCF co-treatment group. The mucosal layer and the crypt layer of the small intestine were thicker in BMMSCs-treated rats, more evident in the BMMSCs-SCF co-treatment group.ConclusionBMMSCs and SCF participate in but may play a synergistic role in mucosal cell regeneration following experimentally induced intestinal injury. Bone marrow stem cell therapy and SCF administration may be of therapeutic value in IBD.

To explore the feasibility of not using antibiotics before and after hemorrhoid surgery is the main objective of the study. A total of 68 patients with hemorrhoids who were admitted to Ningbo Anorectal Hospital from December 2019 to May 2021 were selected, including 36 males and 32 females. They were randomly divided into an experimental group and a control group, 34 patients in each group. The patients in the experimental group were not given antibiotics, while the patients in the control group were given cefazolin sodium 1-2 g and 100 ml of sodium chloride intravenously 30 min before surgery and the same dose of antibiotics was given intravenously after surgery (1 time/12 h), the postoperative administration time is 24 h, not more than 48 h. The intraoperative conditions, postoperative wound infection rate, hospital stay, postoperative recurrence rate and other aspects of the two groups of patients were observed and compared. There was no significant difference in postoperative body temperature, white blood cell count, postoperative wound infection and average hospital stay and recovery time between the two groups. The adverse drug reaction rate in the experimental group was lower than that in the control group. It is safe and feasible not to use antibiotics in the perioperative period of hemorrhoids.

: This is a case report of a 65-year-old female with malakoplakia in the urinary bladder. An ultrasound scan showed many tumor-like polyp lesions, where a transurethral resection was performed. Since the lesions revealed gross papillary neoplasms, malignancy was suspected. However, histpathological examination showed an aggregation of many infiltrating histiocytes containing characteristic Michaelis-Gutmann bodies positively reactive with von Kossa staining, indicating a typical case of malakoplakia of the urinary bladder. Immunohistochemical study demonstrated that the cytoplasms of the histiocytes were focally positive for an anti-Escherichia coli antibody. One of the etiologies of malakoplakia is infection by Escherichia coli, which is supported by our data.