IntroductionGloboid cell leukodystrophy (GLD) is a severe disorder of the central and peripheral nervous system caused by the absence of galactocerebrosidase (GALC) activity. Cell-based therapies are highly promising strategies for GLD. In this study, G-Olig2 mouse embryonic stem cells (ESCs) were induced into oligodendrocyte progenitor cells (OPCs) and were implanted into the brains of twitcher mice, an animal model of GLD, to explore the therapeutic potential of the cells.MethodsThe G-Olig2 ESCs were induced into OPCs by using cytokines and a multi-step differentiation procedure. Oligodendrocyte markers were detected by reverse transcription-polymerase chain reaction (RT-PCR) and immunocytochemistry. The toxicity of psychosine to OPCs was determined by a cell proliferation assay kit. The GALC level of OPCs was also examined. OPCs were labeled with Dir and transplanted into the brains of twitcher mice. The transplanted cells were detected by in-Vivo Multispectral Imaging System and real-time PCR. The physiological effects of twitcher mice were assessed.ResultsOligodendrocyte markers were expressed in OPCs, and 76% ± 5.76% of the OPCs were enhanced green fluorescent protein (eGFP)-positive, eGFP was driven by the Olig2 promoter. The effect of psychosine on cell viability indicated that OPCs were more resistant to psychosine toxicity. The GALC level of OPCs was 10.0 ± 1.23 nmol/hour per mg protein, which was significantly higher than other cells. Dir-labeled OPCs were injected into the forebrain of post-natal day 10 twitcher mice. The transplanted OPCs were myelin basic protein (MBP)-positive and remained along the injection tract as observed by fluorescent microscopy. The level of the Dir fluorescent signal and eGFP mRNA significantly decreased at days 10 and 20 after injection, as indicated by in-Vivo Multispectral Imaging System and real-time PCR. Because of poor cell survival and limited migration ability, there was no significant improvement in brain GALC activity, MBP level, life span, body weight, and behavioral deficits of twitcher mice.ConclusionsESC-derived OPC transplantation was not sufficient to reverse the clinical course of GLD in twitcher mice.

In view of the characteristics of the preparation of an adhesive process which is lengthy, nonlinear, time-varying, big inertia and pure delay, A Proportional-Integral-Derivative (PID) algorithm is proposed based on the improved particle swarm optimization. Because there are drawbacks in the design of PID controller, an improved particle swarm optimization which takes into account a number of performances is propesed to modified parameters of PID controllers. In which the variation of genetic algorithm is introduced to the standard particle swarm optimization algorithm, which can avoid local maximum points, thus the preferable PID controller parameters are easily obtained. Applying the algorithm to the preparation of an adhesive process, the temperature of polymerizing-kettle can be tracked and controlled. Simulation and factual running shows that the algorithm is effective and has excellent performance.

As the pulp consistency system has a big lag behind with uncertain model, the precise control of the pulp consistency could be achieved by making use of the respective advantages of variable domain fuzzy control method and the PID control method based on PSO, and shifting between these two controllers by fuzzy soft handoff switch.

Whereas, colleges of undergraduates have some certain insufficiency in EDA teaching for lack of material theoretical guidance, educating conditions and qualified teachers etc. Guided by the theory of constructivism this thesis developed a study on the criteria and content of EDA technology courses.

The rapid development of information technology has become the important factors that influence economy and people life, automatic control principle course teaching has extensive theoretical and practical value, thus to explore to adapt to the new situation of the automatic control principle course teaching become a problem to be solved. Automatic control theory teaching experiment system based on network was proposed, it is the use of multimedia courseware and the resistance furnace temperature control system is set up network teaching platform, not only more convenient teacher prepares a lesson, class teaching content more rich vivid, but also students can better understand and communicate through the network has been to learn knowledge, and can be learned through simulation test, for the follow-up professional courses to lay a good foundation.