Feline sarcoma-related protein (Fer) is a type of nuclear and cytoplasmic non-receptor protein tyrosine kinase, which is associated with the progression of numerous types of cancer. Previously, we identified that Fer is associated with the migration and invasion of bladder cancer. The present study aimed to investigate the role of Fer in bladder cancer cell viability and apoptosis. reverse transcription-quantitative polymerase chain reaction and western blot analysis were performed to detect the expression levels of Fer; short interference rna (sirna) and overexpression vectors were used to downregulate or upregulate Fer expression, respectively. The effects on cell proliferation ability and cell apoptosis were then tested by MTT assay and flow cytometry. The results revealed that Fer expression was upregulated in bladder cancer cell lines. downregulation of Fer expression by sirna significantly suppressed T24 cell viability and induced apoptosis, as well as inducing cell cycle arrest. conversely, Fer overexpression in 5637 cells significantly promoted cell viability and cell cycle progression, but inhibited cell apoptosis. Furthermore, the suppression and overexpression of Fer significantly altered the expression of cleaved caspase-3 and Bcl-2, and dysregulated the P38 mitogen-activated protein kinase signaling pathway. The findings of the present study indicate a possible molecular mechanism of Fer in bladder cancer and may be considered as a potential target in the treatment of this disease.