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In conclusion, our observations indicate that post-injury treatment with quercetin could inhibit neuronal autophagy and apoptosis in the hippocampus in a rat model of TBI. The neuroprotective effects of quercetin may be related to modulation of PI3K/Akt signaling pathway.

Upper Triassic coal‐bearing strata in the Qiangtang Basin (Tibet) are known to have source rock potential. For this study, the organic geochemical characteristics of mudstones and calcareous shales in the Upper Triassic Tumengela and Zangxiahe Formations were investigated to reconstruct depositional settings and to assess hydrocarbon potential. Outcrop samples of the Tumengela and Zangxiahe Formations from four locations in the Qiangtang Basin were analysed. The locations were Xiaochaka in the southern Qiangtang depression, and Woruo Mountain, Quemo Co and Zangxiahe in the northern Qiangtang depression. At Quemo Co in the NE of the basin, calcareous shale samples from the Tumengela Formation have total organic carbon (TOC) contents of up to 1.66 wt.%, chloroform bitumen A contents of up to 734 ppm, and a hydrocarbon generation capacity (Rock‐Eval S1+ S2) of up to 1.94 mg/g. The shales have moderate to good source rock potential. Vitrinite reflectance (Rr) values of 1.30% to 1.46%, and Rock‐Eval Tmax values of 464 to 475 °C indicate that the organic matter is at a highly mature stage corresponding to condensate / wet gas generation. The shales contain Type II kerogen, and have low carbon number molecular compositions with relatively high C21−/C21+ (2.15–2.93), Pr/Ph ratios of 1.40–1.72, high S/C ratios (>0.04) in some samples, abundant gammacerane (GI of 0.50–2.04) and a predominance of C27 steranes, indicating shallow‐marine sub‐anoxic and hypersaline depositional conditions with some input of terrestrial organic matter. Tumengela and Zangxiahe Formation mudstone samples from Xiaochaka in the southern Qiangtang depression, and from Woruo Mountain and Zangxiahe in the northern depression, have low contents of marine organic matter (Type II kerogen), indicating relatively poor hydrocarbon generation potential. Rr values and Tmax data indicate that the organic matter is overmature corresponding to dry gas generation.

Previous studies have demonstrated that traumatic brain injury (TBI) may cause neurological deficits and neuronal cell apoptosis. Quercetin, one of the most widely distributed flavonoids, possesses anti‑inflammatory, anti‑blood coagulation, anti‑ischemic and anti‑cancer activities, and neuroprotective effects in the context of brain injury. The purpose of the present study was to investigate the neuroprotective effects of quercetin in TBI. A total of 75 rats were randomly arranged into 3 groups as follows: Sham group (Sham); TBI group (TBI); and TBI + quercetin group (Que). Brain edema was evaluated by analysis of brain water content. The neurobehavioral status of the rats was evaluated by Neurological Severity Scoring. Immunohistochemical and western blot analyses were used to measure the expression of certain proteins. The results of the present study demonstrated that post‑TBI administration of quercetin may attenuate brain edema, in addition to improving motor function in rats. Additionally, quercetin caused a marked inhibition of extracellular signal‑regulated kinase 1/2 phosphorylation and activated Akt serine/threonine protein kinase phosphorylation, which may result in attenuation of neuronal apoptosis. The present study provided novel insights into the mechanism through which quercetin may exert its neuroprotective activity in a rat model of TBI.