Surface charge accumulation on insulator surface can have great influence on surface flashover performance. An experimental system is established to investigate surface charge accumulation and decay characteristics of Al 2 O 3 -filled epoxy resin insulators in 0.1 MPa SF 6 under DC voltages. Surface potential is recorded by a Kelvin vibrating probe connected to an electrostatic voltmeter. By pre-depositing charges on insulator surface, the influence of surface charges on surface flashover performance is studied. The results reveal that surface charge distribution appearance is the combined effect of electrode injection, back discharge and gas ionization. Surface charge distribution has obvious polarity effect. It is concentrated near the HV electrode under positive voltages and dispersed under negative voltages. The difference in positive and negative surface flashover voltage is attributed to the difference in surface charge distribution under DC voltages of different polarities. Surface charge decay contains two stages, which satisfies the law of double exponential function. At first stage, surface charge decays fast, which corresponds to charges escaping from shallower traps. While it decays slowly at the second stage, which corresponds to charge escaping from deeper traps. Surface charge decay process is dominated by surface conductivity mechanism. The pre-deposited charges on insulation surface have great influence on surface flashover performance. The deposited positive charges can increase positive flashover voltage but decrease negative flashover voltage.

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A study of the cross-linking of beta-galactosidase on magnetic beads is reported here. The magnetic beads were prepared from artemisia seed gum, chitosan, and magnetic fluid in the presence of a cross-linking regent (i.e., glutaraldehyde). The reactive aldehyde groups of the magnetic beads allowed the reaction of the amino groups of the enzymes. The animated magnetic beads were used for the covalent immobilization of beta-galactosidase. The effect of various preparation conditions on the activity of the immobilized beta-galactosidase, such as immobilizing time, amount of enzyme, and the concentration of glutaraldehyde, were investigated. The influence of pH and temperature on the activity and the stability of the enzyme, both free and immobilized, have been studied. And o-nitrophenyl-beta-D: -galactopyranoside (ONPG) was chosen as a substrate. The beta-galactosidase immobilized on the magnetic beads resulted in an increase in enzyme stability. Optimum operational temperature for immobilized enzyme was 10 degrees Celsius higher than that of free enzyme and was significantly broader.

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A good contact between the pantograph and catenary is critically important for the working reliability of electric trains, while the basic understanding on the electrical contact evolution during the pantograph-catenary system working is still ambiguous so far. In this paper, the evolution of electric contact was studied in respects of the contact resistance, temperature rise, and microstructure variation, based on a home-made pantograph-catenary simulation system. Pure carbon strips and copper alloy contact wires were used, and the experimental electrical current, sliding speed, and normal force were set as 80 A, 30 km/h, and 80 N, respectively. The contact resistance presented a fluctuation without obvious regularity, concentrating in the region of 25 and 50 mX. Temperature rise of the contact point experienced a fast increase at the first several minutes and finally reached a steady state. The surface damage of carbon trips in microstructure analysis revealed a complicated interaction of the sliding friction, joule heating, and arc erosion.