Background Oral microbiome and salivary proteins play a critical role in the occurrence and development of caries. In this study, we used metagenomic and metaproteomic analyses to explore the microbiological and proteinic biomarkers and investigate the etiology of caries in 6–8 years old children. Our study aims to offer a better comprehension of these factors and the relationship with caries, and these findings might facilitate caries risk assessment and provide a basis for future prevention strategies. Methods Children 6 to 8 years old living in rural isolated areas including 40 caries-active subjects and 40 caries-free subjects were recruited. Supragingival plaque and unstimulated saliva were collected for 16S rDNA pyrosequencing and isobaric tags for relative and absolute quantitation (iTRAQ) technique coupled with quantitative nano-flow liquid chromatography-tandem mass spectrometry (LC-MS/MS), respectively. Results We found 6 phyla and 13 genera predominant in all the samples, and differences in relative abundances can be observed. The Alpha diversity analysis demonstrated that the richness and diversity of the bacterial communities were similar between children with caries-free and caries-active groups; LEfSe detected differences in the bacterial community including Dialister, Selenomonas, Actinomyces, and Mogibacterium in the caries-active group (P < 0.05) and Capnocytophaga, Fusobacterium, Desulfuromonadales, Haemophilus, and Porphyromonas in the caries-free group(P < 0.05). The core microbiome was defined as 18 predominant genera in children with caries. The results of the salivary proteome identified 9135 unique peptides and 1662 proteins group from 20 salivary samples. Two hundred fifty-eight proteins were differentially expressed between the caries-free and caries-active groups. Conclusions The diversity of the microbial community has little effect on caries but some bacteria with different relative abundance between the caries-active and caries-free group could be considered as potential biomarkers for children with caries. In addition, as a critical host factor of caries, the salivary proteins are different in caries-free and caries-active groups.

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Labour struggles are frequent in China, but because workers’ organizational resources are controlled by the state, these struggles have been fragmented. Targeting this problem, a group of internationally connected labour NGOs emerged in the Pearl River Delta between 2011 and 2015. These organizations sought to advocate workers’ collective rights by helping workers organize outside the state system. Adopting a relational approach to the study of civil society, this article examines the impact of these NGOs. Based on ethnographic research and a unique data set, it argues that although the organizational skills shared by these NGOs could to some extent sustain workers’ collective actions, they could not be used to integrate the fragmented struggles. Due to the lack of institutional guarantees, activists’ interventions can generate more mistrust than solidarity. The preference of the key donor for a more confrontational and independent labour movement further widened the gap between NGOs and workers, and distracted the NGOs from channels that had the potential to influence policy. The study contributes to an understanding of social movements and NGO intervention by emphasizing the necessity of locating advocacy channels within the state, and the importance of recognizing and maintaining the complex ecology of civil society.

A three-dimensional reconstruction technique based on strain monitoring is proposed in this paper. According to the strain data of finite measuring points, the shape characteristics of the whole rocket structure are reconstructed, which provides the basis for the reliability judgment of the structure. Based on the theory of Ko, the 3D reconstruction algorithm for the rocket model of cylindrical structure is realized. The strain nephogram of plate model is simulated by ANASYS. And the strain nephogram is reconstructed by reconstruction algorithm, then compared with the simulation results. The results show that the maximum error between the results of reconstruction and simulation is 1.91%, which indicated that the agreement is great.

Background Oral microbiome and salivary proteins play a critical role in the occurrence and development of caries. In this study, we used metagenomic and metaproteomic analyses to explore the microbiological and proteinic biomarkers and investigate the etiology of caries in 6-8 year old children. Our study aims to offer a better comprehension of these factors and the relationship with caries, and these findings might facilitate caries risk assessment and provide a basis for future prevention strategies. Methods Children six to eight years old living in rural isolated areas including 40 caries-active subjects and 40 caries-free subjects were recruited. Supragingival plaque and unstimulated saliva were collected for 16S rDNA pyrosequencing and isobaric tags for relative and absolute quantitation (iTRAQ) technique coupled with quantitative nano-flow liquid chromatography-tandem mass spectrometry(LC-MS/MS), respectively. Results We found 6 phyla and 13 genera predominant in all the samples, and differences in relative abundances can be observed. The Alpha diversity analysis demonstrated that the richness and diversity of the bacterial communities were similar between children with caries-free and caries-active groups; LEfSe detected differences in the bacterial community including Dialister, Selenomonas, Actinomyces, and Mogibacterium in the caries-active group (P<0.05) and Capnocytophaga, Fusobacterium, Desulfuromonadales, Haemophilus, and Porphyromonas in the caries-free group(P<0.05). The core microbiome was defined as 18 predominant genera in preschool children with caries. The results of the salivary proteome identified 9135 unique peptides and 1662 proteins group from 20 salivary samples. Two hundred fifty-eight proteins were differentially expressed between the caries-free and caries-active groups. Conclusions The diversity of the microbial community has little effect on caries but some bacteria with different relative abundance between the caries-active and caries-free group could be considered as potential biomarkers for children with caries.In addition, as a critical host factor of caries, the salivary proteins are different in caries-free and caries-active groups.