The SET domain-containing protein, pTAC14, was previously identified as a component of the transcriptionally active chromosome (TAC) complexes. Here, we investigated the function of pTAC14 in the regulation of plastid-encoded bacterialtype RNA polymerase (PEP) activity and chloroplast development. The knockout of pTAC14 led to the blockage of thylakoid formation in Arabidopsis (Arabidopsis thaliana), and ptac14 was seedling lethal. Sequence and transcriptional analysis showed that pTAC14 encodes a specific protein in plants that is located in the chloroplast associated with the thylakoid and that its expression depends on light. In addition, the transcript levels of all investigated PEP-dependent genes were clearly reduced in the ptac14-1 mutants, while the accumulation of nucleus-encoded phage-type RNA polymerase-dependent transcripts was increased, indicating an important role of pTAC14 in maintaining PEP activity. pTAC14 was found to interact with pTAC12/ HEMERA, another component of TACs that is involved in phytochrome signaling. The data suggest that pTAC14 is essential for proper chloroplast development, most likely by affecting PEP activity and regulating PEP-dependent plastid gene transcription in Arabidopsis together with pTAC12.
The present study was conducted to examine the effects of increasing concentrations of chromium (Cr(6+)) (0, 25, 50, 100, and 200 μmol) on rice (Oryza sativa L.) morphological traits, photosynthesis performance, and the activities of antioxidative enzymes. In addition, the ultrastructure of chloroplasts in the leaves of hydroponically cultivated rice (O. sativa L.) seedlings was analyzed. Plant fresh and dry weights, height, root length, and photosynthetic pigments were decreased by Cr-induced toxicity (200 μM), and the growth of rice seedlings was starkly inhibited compared with that of the control. In addition, the decreased maximum quantum yield of primary photochemistry (Fv/Fm) might be ascribed to the decreased the number of active photosystem II reaction centers. These results were confirmed by inhibited photophosphorylation, reduced ATP content and its coupling factor Ca(2+)-ATPase, and decreased Mg(2+)-ATPase activities. Furthermore, overtly increased activities of antioxidative enzymes were observed under Cr(6+) toxicity. Malondialdehyde and the generation rates of superoxide (O2̄) also increased with Cr(6+) concentration, while hydrogen peroxide content first increased at a low Cr(6+) concentration of 25 μM and then decreased. Moreover, transmission electron microscopy showed that Cr(6+) exposure resulted in significant chloroplast damage. Taken together, these findings indicate that high Cr(6+)concentrations stimulate the production of toxic reactive oxygen species and promote lipid peroxidation in plants, causing severe damage to cell membranes, degradation of photosynthetic pigments, and inhibition of photosynthesis.
Soy peptones or soy hydrolysates are widely used as key medium additives in serum-free cell culture processes for industrial production of therapeutic recombinant proteins. The heterogeneous nature of these vegetable-derived materials can lead to substantial lot- to-lot variability in cell culture processes. In this study, we demonstrated the feasibility of nuclear magnetic resonance (NMR) spectroscopy in combination with chemometrics in rapid screening peptone lots in order to optimize efficiency and consistency of large-scale protein production. This report is the first that shows a correlation between the intrinsic NMR spectral characteristics of complex heterogeneous materials and product titer using chemometrics.
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