Previous studies have shown substances capable of similar effects of demineralization, accelerating the process of bone remodeling. This study investigated preosteoblasts behavior in cell culture after bone demineralization with citric acid and tetracycline. Seventy-four Wistar rats provided 144 calvarial bone samples, 126 of which were randomly divided in seven groups according to the treatment given to the surface: no demineralization (C), citric acid (CA), tetracycline (TCN) during 15, 30, and 60 s. Each group received preosteoblasts cultured for 24, 48, and 72 hr. Eighteen remaining samples were analyzed for the atomic percentage (A%) by energy dispersive spectroscopy (EDS) before and after demineralization. The average percentage of bone area covered by cells increased with time and it was significantly higher after 24 and 48 hr of culture in groups CA15s, CA30s, CA60s, TCN15s, and TCN30s than in groups TCN60 and C (p < 0.05). The cell morphology in all CA and TCN groups was shown to be compatible with more advanced stages of differentiation than in C group. The A% changed after demineralization. We conclude that demineralization with citric acid or tetracycline for 15-30 s increased the area of bone surface covered by preosteoblasts. The A% changes were not sufficient to impair the cells spreading and morphology. Bone demineralization may promote potential benefits in bone regenerative procedures. HighlightsLow pH effects did not interfere on cell growth.Bone demineralization favored the preosteoblasts growth.A possible alternative to improve graft consolidation. K E Y W O R D Sbone tissue, cell culture, citric acid, osteoblasts, tetracycline

Background Previous data suggest that bone demineralization may promote bone graft consolidation as well as proliferation and differentiation of pre‐osteoblasts, but the biological mechanisms involved in this process need to be clarified. This study investigated the effects of bone demineralization with citric acid (CA) and tetracycline (TCN) on the repair of onlay bone grafts. Methods Onlay bone grafts were performed on the calvaria of 126 Wistar rats. The contacting surfaces between bone graft and receptor bone bed were demineralized for 15, 30, and 60 seconds with TCN (50 mg/mL), or 10% CA, (pH 1), constituting the following test groups (n = 18): TCN15, TCN30, TCN60, CA15, CA30, and CA60. Control grafts (C) were performed without demineralization (n = 18). After 7, 30, and 60 days, biopsies were obtained for quantitative and qualitative histological analysis (a = 6). Results Demineralization accelerated the bone repair early from 7 days of healing. Higher percentage area of newly formed bone was observed in CA15 and TCN60 groups when compared to C in all evaluation periods (P = 0.02). At 30 days, C specimens had lower percentage of consolidated surfaces than TCN60, TCN30 and CA15 (P = 0.0015). At 60 days, CA15, CA60, and TCN60 presented bone surfaces almost completely filled by newly formed bone, against about 75% in C specimens (P = 0.0015). Conclusions Both CA and TCN were effective in accelerating osteogenesis at the interface between bone grafts and receptor bone beds, especially when applied for 15 seconds and 60 seconds, respectively.

A utilização de implantes dentários tem sido cada vez mais uma escolha por parte dos pacientes. Na busca pela reabilitação unitária ou de todos os elementos dentários, os implantes osseointegrados possuem inúmeros benefícios para o restabelecimento da função, estética, fonética e conforto ao paciente. O objetivo dessa revisão de literatura é abordar a importância dos cuidados pré-operatórios que o Implantodontista deve realizar previamente a instalação dos implantes. Para isso, foi realizada uma busca de estudos nas bases de dados Scielo, Lilacs, PubMed e Science Direct.  com os descritores “dental implants and installation”, “dental implants and complementary exams” com “and” e “or” como ferramentas integrativas de busca. Observa-se diversos estudos abordandos o comprometimento da taxa de sobrevivência e de sucesso de implantes dentários em pacientes com alguma alteração sistêmica. Quando presente essas alterações, o risco de falhas e complicações são maiores, porém a partir de um adequado exame físico e auxiliados por exames complemetares, é possível mimetizar riscos ao paciente e promover mais sobrevida ao implante. Deve se ressaltar que o tratamento com implantes dentais é um procedimento controlado, seguro e confiável, inclusive na reabilitação dos pacientes idosos. Portanto, cabe ao Implantodontodontista possuir conhecimento para saber avaliar integralmente adequadamente o paciente que será submetido a cirurgia de instalação de implantes.

Recently, the use of type I collagen obtained from Nile Tilapia (Oreochromis niloticus) was proposed for the treatment of second and third-degree burning lesions and diabetic ulcers due to its occlusive and healing properties. The aim of this report is to describe the use of Nile tilapia skin as an occlusive barrier to protect palatal wounds after the removal of free autogenous soft tissue grafts. Two patients with a deficiency in the height of keratinized mucosa were indicated for treatment with free gingival grafts. The palatal donor area was covered with a Nile tilapia skin, stabilized by sutures. Seven days after surgery, patients returned for post-operative care. Patients’ reported outcomes were investigated by the use of a visual analogue scale and included pain, discomfort, impact on chewing and speaking. Analgesics consumption was also recorded. Standardized photographs were obtained to monitor wound healing. Patients were followed up for 30 days. Patients reported reduced pain levels, with low consumption of analgesics during the first week after surgery. No discomfort or difficulty in chewing or speaking was reported. No complications such as hemorrhage or edema were observed. These findings suggest that the Nile Tilapia skin may be an interesting alternative as an occlusive biological dressing in palatal wounds harvest of free gingival grafts.