The contribution of different Candida species in oral fungal infections has stimulated the search for more effective therapies. This study assessed the antibiofilm effects of nanocarriers of miconazole (MCZ) or fluconazole (FLZ) on Candida biofilms, and their cytotoxic effects on murine fibroblasts. Three-species biofilms (Candida albicans/Candida glabrata/Candida tropicalis) were formed on 96-well plates, and they were treated with nanocarriers (iron oxide nanoparticles coated with chitosan—“IONPs-CS”) of MCZ or FLZ at 39/78/156 µg/mL; antifungals alone at 156 µg/mL and artificial saliva were tested as positive and negative controls, respectively. Biofilms were analyzed by colony forming units (CFU), biomass, metabolic activity, and structure/viability. The cytotoxicity (L929 cells) of all treatments was determined via 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) reduction assay. Data were submitted to one- or two-way ANOVA, followed by Tukey’s or Fisher LSD’s tests (p < 0.05). IONPs-CS-MCZ at 78 µg/mL promoted similar antibiofilm and cytotoxic effects compared with MCZ at 156 µg/mL. In turn, IONPs-CS-FLZ at 156 µg/mL was overall the most effective FLZ antibiofilm treatment, surpassing the effects of FLZ alone; this nanocarrier was also less cytotoxic compared with FLZ alone. It can be concluded that both nanocarriers are more effective alternatives to fight Candida biofilms compared with their respective positive controls in vitro, being a promising alternative for the treatment of oral fungal infections.

O objetivo do presente artigo foi relatar uma forma de confecção de prótese total utilizando a clonagem da prótese provisória com a finalidade de reduzir as etapas do tratamento. Paciente desdentada total superior e classe I de Kennedy inferior compareceu ao consultório apresentando inflamação severa do tecido periodontal, mobilidade grau III de todos os dentes e supuração à sondagem. Optou-se por realizar prótese total superior convencional e realização das exodontias dos dentes remanescentes, seguida de prótese total provisória e como tratamento definitivo mandibular uma prótese total convencional. Na primeira sessão foi realizada a reavaliação dos tecidos bucais, moldagem da prótese total superior para obter o modelo antagonista, seleção da cor do dente, registro para montagem no articulador semi-ajustável e a clonagem da prótese total inferior provisória. Após polimerizada, a réplica em resina acrílica incolor foi ajustada em boca e realizada a moldagem de borda com godiva em bastão, seguida da moldagem funcional com silicone de condensação. Ambos modelos foram montados no articulador para montagem dos dentes artificiais. A prova estética e funcional foi realizada na segunda consulta e na terceira a prótese foi instalada junto com as instruções de uso e higiene oral. Os controles foram realizados após 24 horas, 3, 7 e 14 dias. O uso de uma técnica alternativa para confecção de uma moldeira individual pode reduzir o tempo clinico, trazendo assim vantagens tanto para o paciente como para o profissional, desde que haja um bom planejamento e conhecimento da técnica.Descritores: Prótese Total; Técnica de Moldagem Odontológica; Materiais para Moldagem Odontológica.ReferênciasEmami E, Souza RF, Kabawat M, Feine JS. The impact of edentulism on oral and general health. Int J Dent. 2013;2013:498305Williams SE, Slice DE. 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