This study examined the applicability of gin waste for litter use in broiler production. 630 day-old Ross 308 hybrid birds were used in three treatment groups with three replications kept on commonly used wood shavings (L1), on gin waste (L2) and on a combination of 50% wood shavings and 50% gin waste (L3). During the 42-day experiment, changes in live weight, feed intake, feed conversion rate, vitality, carcass characteristics (weight, yield, gizzard, liver and heart weight), moisture content and enumeration of Eschericia coli in the bedding materials were examined. The study revealed that the effect of litter materials was significant on live weight and carcass weight (P<0.05). The best results regarding live weight and carcass weight (2177.93 g and 1643.10 g) were gained in L2, followed by L1 (2173.27 g and 1622.70 g), and L3 (2086.23 g and 1544.70 g). No statistically significant differences were found for liver, heart and gizzard. The differences in feed consumption and feed conversion ratio were statistically not significant. No statistically significant differences were found for vitality. At study end, moisture content was statistically different in L2. In L1 and L3, E coli colonization was determined, while it was not observed in L2. As a result, it can be concluded that gin waste is an alternative litter material to be used in broiler breeding.