Antifungal metabolites were isolated from a culture of Pseudomonas aurantiaca IB5-10. Chemical structures of the metabolites were elucidated as phenazine-1-carboxylic acid (PCA; 1), 2-hydroxyphenazine (2-OH-PHZ; 2), and cyclo-(L-Pro-L-Val; 3), respectively, based on spectroscopic methods. Among them, 3 was isolated for the first time from this strain. The antifungal activities of 1-3 were evaluated against a variety of plant pathogens. To the best of our knowledge, the antifungal activities of 3 against plant fungal pathogens have been evaluated for the first time in this work. PCA (1) showed the most potent antifungal activities against Phytophthora capsici, Rhizoctonia solani AG-1(IA), and Pythium ultimum with MICs (µg/ml) of less than 1.0, 1.3, and 2.0, respectively. On the other hand, 2-OH-PHZ (2) showed potent antifungal activity against R. solani AG-1(IA) with the MIC (µg/ml) of 2.0, whereas it showed moderate antifungal activity against P. ultimum with the MIC (µg/ml) of 50.0. In addition, 3 showed antifungal activity against only R. solani AG-1(IA).

To investigate the phytochemical constituents of Hibiscus hamabo, a chromatographic method was used. A new phenolic glucoside, woodorien A (1), along with 10 known compounds, rutin (2), isoquercetin (3), nicotiflorin (4), astragalin (5), trans-tiliroside (6), N-trans-feruloyltyramine (7), N-trans-caffeoyltyramine (8), woodorien (9), quercetin 3 0 -galactopyranoside (10), and cis-tiliroside (11), was isolated. The structure of 1 was identified as ethyl 3-O-β-D -glucopyranosyl-4-hydroxybenzoate. Compounds 8, 9, and 11 were isolated from genus Hibiscus, and 2-7 and 10 were isolated from H. hamabo for the first time. Their chemical structures were determined through the interpretation of spectroscopic data. The properties of the UVA photoaging induction assay of all isolated compounds were measured. Compounds 3, 8, and 10 exhibited recovery from photoaging induced by UVA in CCD-986sk. These results suggest that H. hamabo is a potential source of natural antiphotoaging ingredients and can be used in cosmetic applications. K E Y W O R D S antiphotoaging, ethyl 3-O-β-D -glucopyranosyl-4-hydroxybenzoate, Hibiscus hamabo, phenolic glucoside, woodorien A