In the present study , 32 white mice Balb /C (two months age ) were used ,therewere divided into four equal groups . Three groups were injected with fungalsuspention of candida albicans in doses of 1x106 ,1x108 and 3x108 cell/ml,whereas the fourth group was regarded as control and injected with 0.5 mlnormal saline solution . All were dissected after 6 days of injection to study thephysiological effect of the yeast on blood , which include RBCand WBC count ,Hb concentration and PCV ratio . And on biochemical content whichinclude the determination of protein , glycogen and cholestrol in liver ,kidneyand muscles .The results showed that the number of RBC were decreased ,while WBCwere increased in the injected mice ,but there were no signifficant effect onPCV and Hb . On the other hand ,cholestrol values were decreased significantlyin the injected mice in comparison with control,but this decrease was varied indifferent tissues (P>0.01).The value was lower in liver ,whereas no signifficantdifference was found between Kidney and muscles . Protein values were notsignifficantly different in comparison with control , but it was different amongtissue studied .muscles was the highest value and the liver was lowest .Glycogen decreased signifficantly in comparison with control ,but not amongtissues.

Background: Even though intra-abdominal candidiasis (IAC) has been increasingly recognized, with associated high morbidity and mortality rates, its pathogenesis remains poorly understood. This model aims to study the pathogenicity and invivo susceptibility of non-albicans Candida species associated with IAC in human in order to predict the frequency of infections, outcome of clinical disease and response to antifungal therapy. Methodology: Both immunosuppressed and immunocompetent female CD-1 mice were challenged intraperitoneally with 5 x 10 8 CFU/ml inoculum of five non-albicans Candida strains; Candida glabrata, Candida parapsilosis, Candida lipolytica, Candida tropicalis and Candida guilliermondii. Mice were closely observed for symptoms. Treated groups received voriconazole (40 mg/kg/day) or micafungin (10 mg/kg/day) 24 hours after infection depending on invitro susceptibility results. Survival rate, mean survival time and fungal tissue burdens were recorded for all groups. Results: All infected groups developed hepatosplenomegaly, peritonitis and multiple abscesses on intra-abdominal organs and mesenteries. C. glabrata and C. lipolytica represented the most and the least virulent strains respectively in terms of survival rate, mean survival time and fungal burden in both immunosuppressed and immunocompetent models. Following treatment, all immunocompetent animals survived the entire duration of experiments (0% mortality rate), while mortality rate was relatively high (20-60%) in immunosuppressed mice. Treatment failed to eradicate the infection in immunosuppressed mice despite significant decrease of the fungal burden and increase mean survival time. Conclusion: This study reports an increasing pathogenicity of non-albicans Candida species, with persistent infection among immunosuppressed animals.