The aim of this research is to observed the effect of starter dosage and fermentation time on pH and lactic acid production. This study used Completely Randomized Design (CRD) factorial pattern 5 x 3. The first factor is starter dosage and the second factor is fermentation time. Kefir samples were prepared using of 5, 10, 15, 20 and 25% (v/v) kefir grains, with three fermentation time 8, 16 and 24 hours. Results indicated that kefir with 10% starter and 16 hours fermentation time give the best results in pH (3.95) and lactic acid production (1.476%).

180 broilers, five weeks old broilers were used to asses the effect of transportation on broiler meat pH and tenderness, has been carried in Bandung, Indonesia. Using Completely Randomized Design (CRD), with five levels (P-0 slaughter at the farm, P-1 one hour transportation before slaughter, P-2 two hours transportation before slaughter, P-3 three hours transportation before slaughter, and P-4 four hours transportation before slaughter) and four times replications. Results indicated the meat pH are between 6.33-6.63. Even the result shows that the pH values increase when the transportation time more longer, but this results are between the normal meat pH (between 5.2-6.6). The meat tenderness were between 124.75-176.50 mm/g/10 sec. The meat tenderness was decreased when the transportation was prolonged.

Hundred day old chicks Arbor Acres CP-707 were used randomizedly in this experiment, to study the effects of various levels skipjack tuna bone meal in ration on broiler carcass tenderness and abdominal fat, and were studied for six weeks. Research using Completely Randomized Design (CRD). The dietary treatments are: R 0 basal diet as control, R 1 basal diet + 2% tuna bone meal, R 2 basal diet + 4% tuna bone meal and R 3 basal diet + 6% tuna bone meal, and each treatments were repeated five times. Results indicated that the highest carcass tenderness was get from the broiler that fed basal diet with 6% tuna bone meal (125,4 mm/g/10sec) and the lowest was get from the broiler that fed basal diet (107 mm/g/10sec). For the abdominal fat, the results is broiler that adding skipjack tuna bone meal in the ration will give more abdominal fat to the broiler (1.89%-1.92%) versus 1.85% for basal diet.

Using chemical bio-preservatives is being frowned at because of their probable adverse effects on the health of consumers. Isolation and identification of microorganisms from natural resources are an occurring process that have most powerful means for obtaining cultures and also have commercial purposes. The aim of this study was to get bio-preservatives from poultry meat, by isolation and then identification of lactic acid bacteria. Lactic acid bacteria do not pose any health risks to human, and are generally recognized as safe (GRAS) organisms. The lactobacillus were isolated from raw poultry meat by appropriate dilutions with NaCl fisiological, and the decimal dilution were mixed with MRS medium and then incubated at 370C for 48-72 h. Pure cultures were maintained in MRS broth agar at 40C for short term use. Thirty well-isolated colonies were picked up and transferred to MRS broth. Selection of strains was made in agreement with morphology, Gram-stain, viability during storage at 40C and antimicrobial activity, was found twenty isolate. The identification of the cultures was based on the characteristics of the lactobacilli as described in Bergey’s manual of determinative bacteriology, fermentation of different carbon sources, gas production from glucose, growth at different temperatures. For anti-biogram, the isolates were inoculated into MRS broth individually and incubated for 24h. The plates were incubated at 37oC overnight. Resistance was defined as the absence of a growth inhibition zone around the discs. Results indicated that 20 isolate of Lactic acid bacteria were identified: 3 isolates of Lactobacillus fermentum, 2 isolates of Lactobacillus paracasei ssp. paracasei, 5 isolates of Lactobacillus plantarum, 3 isolates of Lactobacillus rhamnosus, 2 isolates of Lactobacillus lactis ssp. lactis 1, and   5 isolates of  Lactobacillus lactis ssp. lactis 2. Characterization of the microbial metabolic product for antimicrobial agents reveals that lactic acid bacteria has responsibility for the inhibition of the indicator organisms, and can be  used as meat biopreservator. Keywords: isolation, identification, lactobacillus, poultry meat, biopreservator.

Adulteration of meatballs using pork begins to bloom in a row with the increase of beef price in the market. There is a risk that can occur around the education area of Jatinangor. Therefore the identification of meatballs adulteration with enzymes linked immuno sorbent assay (ELISA) approach need to be done in order to create certainty and security guarantee of the consumed products. Results showed that Small Medium Enterprises (SMEs) in Jatinangor who process meat (meatballs) was 21 meatball traders around Jatinangor. ELISA tests on 21 meatball samples taken from the SMEs merchant in Jatinangor showed no pork-adulterated meatballs on all of the samples tested.