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Fourier transform infrared studies on Langmuir-Blodgett Films of glucose oxidase (GOD) incorporated into fatty acid multilayers have been used to study molecular order and interactions as a function of dipping pressure on the subphase. The data show that GOD is incorporated principally by electrostatic (as opposed to hydrogen-bonded) forces and that the enzyme does not penetrate the acid head group region. Changes of conformation as a function of surface pressure may easily be monitored.

SynopsisThe hydrolysis of ( SN), in air at room temperature and 90% relative humidity has been studied using the attenuated total reflectance ( ATR) method. Decomposition gave rise to strong bands at 3210 and 3150 cm-' [ v3 and v1 (NH:)], 1420 cm-' ( v b NH:), 1220 cm-' ( S = O ) , 1089 and 610 cm-' [ u1 and v3 ( S O : -) ] . For the first 3 days, the decay of the 808 and 690 cm-' bands of (SN), was first order, with a half life of about 30 h. The spectroscopic data were consistent with the rapid formation of -S02-NH2 and =S=NH chain end groups with subsequent relatively slow hydrolysis to ( NH4)zS04, sulfur, and fresh hydrolysable chain ends.