Twenty-three polymorphic microsatellite loci were developed from the black rockfish, Sebastes schlegelii, with an enriched partial genomic library by magnetic beads and polymorphism of these loci was assessed in 32 individuals from a wild population. The loci yielded 2-19 alleles per locus, the observed, expected heterozygosity and polymorphic information content ranged from 0.063 to 1.000, 0.091 to 0.945 and 0.085 to 0.926, respectively. Twenty loci confirmed to Hardy-Weinberg equilibrium and only one pairs of loci show significant linkage disequilibrium after Bonferroni's correction. The availability of these markers will facilitate studies of the conservation genetics of S. schlegelii.