Conclusions:We demonstrated in the present study that the lower serum levels of PIICP, that indicate the reduction of cartilage synthesis, were associated with the lower JSW, that is an one of the typical signs of OA. We speculate that the joint space narrowing could occur when the cartilage repair system is burned out according to the lower cartilage synthesis potential. In conclusion, the lower cartilage synthesis was associated with the radiographic joint space narrowing of the knee joint in men in early forties.Purpose: To assess the effect that mangiferin(1), a standard component of WIN-34B, has on chondrogenic differentiation of mesenchymal stem cells(MSCs) from rabbit subchondral bone. Methods: Histological analysis was performed by alcian blue, oil red O, and alizarin red staining to evaluate if progenitor cells from rabbit subchondral bone have chondrogenic, adipogenic, and osteogenic differentiation capacity. Changes in levels of chondrogenic markers type II