Objective: The purpose of the present study is to develop, statistically optimize and evaluate the transfersomal gel formulation of Tofacitinib citrate via transdermal route by DOE Approach. Materials and Methods: Transferosomes are made up of with bilayer forming phospholipids and biocompatible surfactants like sodium deoxycholate. The surfactants also acts as edge activator which stabilizes the phospholipid bilayer and increases the deformability of the vesicle. The formulation were designed by Box-Behnken Design. For usage as a transferosomal gel, drug encapsulation in various transfersomal formulations having 100 mg drug concentrations and Carbopol-934 (0.5,1.2 g) is being studied. Results: Entrapment efficiency (EE percent), drug content, in-vitro skin penetration testing, and stability studies were all found in the produced formulations. Transmission Electron Microscopy confirmed that the vesicles were spherical in shape. According to the findings, Tofacitinib citrate was effectively pinned with a standardised drug concentration in all formulations. The 0.1 g Tofacitinib citrate optimised transferosome formulation TG2 exhibited encouraging results, with maximum drug release (94.32%). Conclusion: Transferosomes are a promising long-term delivery route for Tofacitinib citrate and are relatively stable. This research work reveals that transferosomes containing Tofacitinib citrate could be used to treat squamous cell carcinoma via transdermal drug delivery.

Objective: The focus of this research was out whether a transfersomal gel formulation for transdermal delivery of Metronidazole. Methods: Azole Antifungals include metronidazole, which is used to treat fungal and yeast infections. Transferosomes are supra-molecular aggregates that are ultra-flexible and have a high ability to penetrate mammalian skin intact. Drug encapsulation in various transfersomal formulations containing various ratios of different drug concentrations (0.05, 0.1, 0.2, 0.3, 0.4, 0.5 g) and Carbopol-934 (0.5, 1, 2 g) is being researched for use as a transferosomal gel.Results: Entrapment efficiency (EE %), drug content, in-vitro skin permeation tests, and stability investigations were performed on the produced formulations. The vesicles were spherical in shape, as confirmed by Transmission Electron Microscopy. Metronidazole was successfully pinned with a standardised drug content in all formulations, according to the resultsThe 0.1 g Metronidazole optimised transferosome formulation MG2 showed promising results, with maximum drug release (94.32%) and maximum drug release (94.32%). Conclusion: According to this report, transferosomes are a promising long-term delivery mechanism for metronidazole and have reasonably good stability.This study suggests that transferosomes containing Metronidazole may be used as a transdermal drug delivery tool for fungal skin infections.