Ectoparasites on wild birds and mammals threaten health of the animal and community. Most studies of ectoparasites tend to be location focused so characterizing wider differences between ectoparasites and hosts is difficult. The aim of this study was to identify ectoparasites from free ranging and captive wildlife at an academic zoological medicine service in Central Oklahoma and record first reports of ectoparasites and hosts. We collected 228 ectoparasites from 47 animals: 111 ectoparasites from 30 birds were 14 lice (n = 78), four hematophagous fly (n = 20), and three tick (n = 13) species. One hundred seventeen ectoparasites from 17 mammals were four flea (n = 8) and four tick (n = 109) species. Results included 13 first-time ectoparasite species and 26 first-time host-ectoparasite reports in Oklahoma. The study portrays closer representation of ectoparasites infesting wild animals in Central Oklahoma and demonstrate possible correlation between mortality and injury to parasitized hosts. Additional studies would document ectoparasites infesting wildlife in Central Oklahoma.