The experiment was conducted under the agro-ecological zone of Modhupur Tract (AEZ-28) to determine the genetic diversity among 39 rice genotypes, collected from Bangladesh Rice Research Institute, Lal Teer Seed Ltd. and Chittagong Hill Tracts. The genotypes were grouped into six clusters based on Mahalanobis's D 2 statistics and Canonical Variate Analysis. Cluster I consisted with the highest number of (11) genotypes from different origin, whereas cluster IV consisted with the lowest number of (3) genotypes. Cluster III consisted of BRRI dhan 28, BRRI dhan 45, Gold, Tia, Dowel and Moyna showed maximum mean yield. Maximum inter cluster distance was recorded between cluster II and cluster III. The highest inter genotypic distance was observed between Tharak dhan and BRRI dhan 45. Cluster III had the highest mean values for grain yield, harvest index, 1000-grain weight and number of effective tillers per hill and cluster V for number of primary branches per panicle and cluster VI for number of filled grains per panicle that had significant positive correlation with grain yield. Number of unfilled grains per panicle, 1000-grain weight and grain yield showed maximum contribution towards genetic divergence. The genotypes from these clusters with desirable characters may be used as potential donor for future hybridization program to develop high yielders.
Random Amplified Polymorphic DNA (RAPD) markers were used to study the molecular diversity of 12 popular potato varieties in Bangladesh. DNA was extracted from tender leaf sample for PCR amplification. The PCR amplified DNA profile was visualized on 2% agarose gel, staining with ethidium bromide. Eight RAPD primers were used to evaluate the genetic diversity of potato varieties. Some total of 36 DNA fragments were amplified and out of them 24 were polymorphic. Those primers generated 61.53% of polymorphic DNA band. The primer OPX 04 produced highest (9) number of DNA band and out of 9 amplicon 6 were polymorphic. Lowest number of amplification was observed in the primer OPA-17 and it was only 3. The highest Nei's genetic distance (0.9701) was noticed between the variety Granola and Provinto. The highest (0.8205) number of genetic identity/similarity was observed between the varieties Cardinal and Diamant. The unweighted pair group method of arithmetic mean (UPGMA) dendrogram based on Nei's genetic distance revealed that the 12 varieties followed into two main clusters. The present finding showed that there was high level of genetic diversity among the varieties which can be used for parental selection in potato breeding program.
The present research was carried out in Biotechnology Laboratory of the Department of Biotechnology, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka-1207 from the period of September 2017 to June 2018. This research aims to study the effect of Benzyladenine (BA), Kinetin (KIN) and Naphthalene acetic acid (NAA) either in combination or alone on In vitro propagation of papaya (Carica papaya). The shoot tips of young shoots were used as explant, which was sterilized using freshly prepared 0.1% HgCl2 mixing with few drops of Tween-20, were inoculated in MS media supplemented with 0.1% activated charcoal. The minimum days to shoot induction (10.25) were recorded on MS medium containing 0.5 mg/L BA. The highest shoots (4.5) and length of shoot (5.75 cm) observed in 1.0 mg/L BA. The combined treatment 1.0 mg/L BA+0.75 mg/LKIN gave the highest number of shoots (5.25) and length of shoot (5.78 cm).The minimum days (8.5) to root induction was reported in 2.0 mg/L NAA along with maximum 8.25 roots per plantlet. The highest length of root (6.92 cm) was observed in 2.0 mg/L NAA. In regenerated plantlets, 80% survival rates were observed in growth chamber conditions and 75% in the open atmosphere were achieved. Finally, the in vitro regeneration protocol described herein can potentially be used as a tool in molecular breeding programs for the improvement of different cultivars and genotypes of papaya.
Twenty diverse rice germplasm were used to estimate genetic diversity using five microsatellites (SSR) and five randomly amplified polymorphic DNA (RAPD) markers. A total of 33 clear and repeatable bands were scored from RAPD markers and 33 alleles were detected from SSR primer pairs. Average polymorphic information content (PIC) value across the SSR alleles was 0.789 and average polymorphism for all microsatellite markers was 100%. Polymorphism level revealed by RAPD was 93.80%. The unweighted pair group method of arithmetic means (UPGMA) dendrogram on Nei's genetic distance based on SSR markers clubbed germplasms into three clusters:
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