The effects of light quality and irradiance, and supply of organic carbon and vitamins on the growth of two forms of Ecklonia radiata in tissue culture were examined. A callus of unpigmented cells developed over the cut surface of newly excised explants of stipe. This growth was best in the dark but stopped after 10 weeks. Pigmented, mainly filamentous clumps of cells developed from explants after several weeks in culture. These required light for growth, with growth being enhanced by increasing photon flux density up to 30 /mol photon m-2 s -, with the active spectral component being red light (> 600 nm). The addition to the medium of a range of organic carbon sources or vitamins did not stimulate growth of either culture type in the dark.

Sections of the thallus of the brown alga Ecklonia radiata (C. Ag.) J. Ag. were surface sterilised for tissue culture by dipping in 70% ethanol for 30 s, followed by sterile deionised water for 30 min. A high percentage of aseptic viable explants could be obtained using this treatment, and growth in vitro was initiated.

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