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Forty children allergic to grass pollen were divided into two groups, and matched by their serum IgE antibody concentrations to grasses. For two preseasonal treatment periods one group received immunotherapy with an alum adsorbed grass pollen extract, while the second group was treated orally with an aqueous extract of the same allergens. The patients' postseasonal self evaluation as well as the mean symptom scores calculated from symptom diaries indicate, that oral treatment is significantly less effective in controlling seasonal allergic symptoms than subcutaneous immunotherapy. During oral treatment neither an increase of specific serum IgG antibodies, nor a suppression of the seasonal increase in specific serum IgE antibodies could be demonstrated.

Protein values of dialysed allergen extracts determined by Lowry, modified Lowry (trichloroacetic acid precipitation of the proteins) and dye-binding assay were compared. The influence of different parameters on the modified Lowry was examined. The reproducibility of the modified Lowry was checked with three independent measurements. For the examination of recovery a constant amount of 6-grass pollen allergen proteins was added to the samples of the standardized human serum albumin prepared for the calibration curve. The samples were measured by modified Lowry. The mean of the ratio between the protein values of the dialysed allergen extracts obtained by modified Lowry and those obtained by classical Lowry was 3.59 (coefficient of variation C v = 45%). The mean of the ratio between the protein values of the allergen extracts obtained by modified Lowry and dye-binding assay was 1:0.71(C V = Phenol interfered with the modified Lowry. Phenolic allergen extracts showed higher "protein values" than non-phenolic allergen extracts. This influence could be reduced by a second precipitation of the dissolved precipitate. The precipitation of non-phenolic dialysed aqueous allergen extracts was complete after the first trichloroacetic acid precipitation. By incubating samples with the Folin-Ciocalteu's reagent at 55 °C in a waterbath, the time necessary for developing the colour could be reduced from 45 min to 5 min. Protein measurements by modified Lowry of a 6-grass pollen allergen extract in three different laboratories showed good reproducibility. For these extract 785 Mg protein/ m/ (C v = 4%) could be measured. The addition of a constant amount of allergen proteins to the samples prepared for the human serum albumin calibration curve produced a curve parallel to this calibration curve. Vergleich der klassischen und der modifizierten Proteinbestimmung nach Lowry und des "dye-binding assay" zur Analyse von Allergenextrakten und Untersuchung des Einflusses von unterschiedlichen Bedingungen auf die Proteinbestimmungsmethode nach dem modifizierten Lowry-TestZusammenfassung: Die Proteinwerte von dialysierten Allergenextrakten wurden mit der Proteinbestimmungsmethode nach Lowry, modifiziertem Lowry-Test (Trichloressigsäurefällung der Proteine) und "dye binding assay" ermittelt und miteinander verglichen. Der Einfluß von unterschiedlichen Versuchsbedingungen auf den modifizierten Lowry-Test wurde untersucht. Die Reproduzierbarkeit des modifizierten LowryTests wurde in drei unabhängigen Messungen überprüft. Zur Durchführung eines Wiederfindungsexperimentes wurde eine konstante Menge Protein eines 6-Gräserpollen-Allergenextraktes zu den Proben gegeben, die für die Humanserumalbumin-Kalibrierungskurve hergestellt wurden. Der Mittelwert des Verhältnisses der Proteinwerte, der bei den dialysierten Allergenextrakten mit dem modifizierten und dem klassischen Lowry-Test gemessen wurde, betrug l: 3.59 (Variationskoeffizient C v = 45%). Der Mittelwert des Verhältnisses der Proteinwerte, der bei den Allergenextrakten mit dem modifizierte...

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