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The aim of this study was to isolate and characterize the Phosphate solubilizing bacteria from the rhizosphere of Zea mays L., Jeneponto Regency. This research was conducted in several stages; i.e, sampling, medium preparation, sample dilution, isolation, characterization in the form of gram staining, biochemical tests, and quantitative tests of phosphate solubility. Soil samples were diluted in 0.9% NaCl and soil containing microbes was isolated on the Picovskaya medium. Three isolates were obtained which could dissolve phosphate, namely J2KN1, J3KR2, and J3TG3 isolates. The isolates were generally round in shape with raised elevations, white, slimy, smooth, shiny surface, milky white, shape like coccus and bacillus, and gram-negative. Some of the isolates had positive motility, indole, voges, methyl red, glucose, and sucrose fermentation in the biochemical test. The quantitative tests of the ability to dissolve phosphate showed that J2KN1 isolate had the highest concentration of 51.1 μM, and the J3KR1 and J3TG3 isolates had a concentration of 45.2 μM and 37.6 μM, respectively.

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The importance of essential metal ions and their metal complexes in the creation of prospective medical therapies has long been recognized. In chemistry, molecular biology, and medicinal fields; the interaction of metal complexes with DNA has been a subject of study. The dithiocarbamate essential metal complex is described extensively in the literature for its various benefits and advantages. With proper use of ligands, it is proven to increase the cytotoxic activity of metal complexes against cancer cells. Some researches have shown significant progress regarding the biological activities of the dithiocarbamate essential metal complex as antimicrobial, antioxidant, and anticancer agents. Metal complexes form complexes with dithiocarbamate ligands with unique structural variations. In this study, we presented an overview of the cytotoxic effects of some dithiocarbamate essential metal complexes on cancer cells, as well as fresh approaches to the design of essential metal-based therapeutics containing dithiocarbamate and molecular targets in cancer therapy. This review may provide an update on recent developments in the medicinal use of essential metals with dithiocarbamate ligands, carried out to identify recent relevant literature. Finally, we predict that the essential metal complexed with dithiocarbamate can be a new breakthrough in the future development of cancer drugs.

Increased resistance to TB drugs, may render vaccine development a more effective approach to stop or reduce TB epidemics. The antigen Culture Filtrat Protein Filtrat 21 (CPF21) is an immudominant protein encoded in RD 2 region of the Mycobacterium tuberculosis genome, capable of obtaining a strong hypersensitivity reaction and to induce very high interferon-gamma (IFN-γ) responses in patients with tuberculosis. In order to construct the recombinant plasmid pGEM-T Easy-CFP21 and express it in E. coli BL21, the CFP21 gene was amplified from M. tuberculosis H37Rv genomic DNA using PCR in vitro, and inserted into the pGEM-T Easy cloning vector. The recombinant plasmid was then transformed into E. coli JM109, followed by plasmid extraction, PCR amplification, and DNA sequencing. The correct recombinant CFP21 gene was subcloned into expression vector pGEX-2TK and transformed into E. coli BL21 strain. The white recombinant colony was selected, cultured, induced with 50 µM IPTG, and identified using SDS-PAGE electrophoresis method. These results demonstrated that CFP21 gene has been constructed and expressed successfully. The molecular weight was about 47 kDa as the fusion protein GST-CFP21 and expressed as insoluble protein. In conclusion, the target gene CFP21 has been cloned into host E. coli BL-21 strain and expressed successfully. In the future, the purified recombinant fusion protein GST-CFP21 paves the way for TB diagnosis and vaccine development.