Highlights d Physcomitrella patens provides an early evolutionary snapshot of 11 KAI2-like proteins d A loop segment determines substrate specificity in Physcomitrella KAI2-like proteins d Distinct groups of proteins perceive (À)-5-deoxystrigol and the karrikin KAR 1 d Physcomitrella KAI2-like proteins cannot complement Arabidopsis kai2 or d14 mutants
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