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a b s t r a c tThree substerilizing doses 50, 100 and 150 Gy of gamma radiation were tested against full e grown male and female pupae or against full-grown male or female pupae of Agrotis ipsilon.The results showed that fecundity of irradiated females crossed with irradiated males was decreased by increasing irradiation dose. The decrease in egg e hatchability % and increase in sterility % induced by gamma radiation were found to be positively correlated with the dose level. The parentage of larval and pupal mortality increased significantly (p 0.05) with the increase of used doses. In addition, larval and pupal durations were found to be significantly prolonged as a result of gamma-irradiation treatment. In general, the results obtained indicated that the biological action of gamma irradiation against A. ipsilon larvae was more remarkable when both crossed females and males were irradiated followed by irradiated females crossed with non-irradiated males.

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Combined effect of substerilizing doses of gamma radiation (40 and 100 Gy) and different concentrations of the entomopathogenic nematode, Steinernema carpocapsae BA2 (20 and 40 IJs/ml) on the hemocyte count of the greater wax moth, Galleria mellonella (L.) larvae was studied. Eight types of hemocytes were described in the hemolymph of the normal larvae of G. mellonella. Prohemocytes were the predominant type, while the cystocytes were the rare ones. Morphological malformations and changes in the number of each hemocyte type were observed in F1 larvae (of irradiated male parent pupae with 40 or 100 Gy) or larvae treated with different concentrations of the S. carpocapsae BA2 (normal or F1 larvae); these alterations were increased by increasing the radiation dose or the nematode concentration that led to increase the susceptibility of the larvae to the nematode. Therefore, it could be concluded that integration of entomopathogenic nematodes and gamma radiation may serve as integrated control program for G. mellonella.

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