Background/purpose Plasma rich in growth factors (PRGFs), which is prepared from autologous blood from patients, has been reported with regards to bone regeneration for dental implants. Human dental follicle cells (hDFCs) have the capacity to commit to multiple cell types such as the osteoblastic lineage. The aim of this study is to evaluate the effects of PRGFs for mineralization in hDFCs. Materials and methods PRGFs was prepared from whole blood centrifuged at 460 g for 8 minutes. hDFCs isolated from the dental follicle with collagenase/dispase were cultured with growth medium or osteogenic induction medium (OIM) containing PRGFs or fetal bovine serum. Concentrations of the growth factors were examined using an enzyme-linked immunosorbent assay kit. A cell migration assay was used for two-dimensional movement. Gene expressions were examined with real-time polymerase chain reaction using a DyNAmo SYBR Green quantitative polymerase chain reaction kit. Results The platelet concentration in PRGF Fraction 2 was 2.14-fold higher than in whole blood. White blood cells were not detected in PRGFs. Transforming growth factor-β levels were higher than insulin-like growth factor-1, platelet-derived growth factor-AB and -BB, and vascular endothelial growth factors in PRGF Fraction 2. Proliferation and migration by hDFCs increased in OIM supplemented with PRGFs in a dose-dependent manner and were higher in hDFCs cultured in OIM plus 10% PRGFs compared with OIM plus 10% fetal bovine serum. PRGFs upregulated the gene expression of type I collagen , osteomodulin , alkaline phosphatase , bone morphogenic protein-4 , and transforming growth factor-β in hDFCs. Conclusion PRGFs may promote bone regeneration due to it including high levels of growth factors.

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with facial asymmetry and jaw deformity affected the longterm stability and reversion of the mandibular position (3). Furthermore, Kai et al examined mandibular changes after SSRO and reported that these changes may be due to factors such as postoperative occlusion, muscle position, morphology of condyle and the glenoid Correspondence to：

Plasma rich in growth factors(PRGF)can be rapidly obtained from patient blood. PRGF has been used in regenerative therapy for soft tissue and bone formation, and represents a new and potentially useful adjunct in oral and maxillofacial bone reconstructive surgery. However, few studies have investigated the biological functions of PRGF in bone regeneration. Human mesenchymal stem cells(hMSC)isolated from human bone marrow have the capacity to commit to multiple cell types such as the osteoblastic lineage, and have been widely applied in tissue engineering studies in recent years. The aim of this study is to evaluate the effects of PRGF for osteogenic differentiation in hMSC. PRGF was prepared from whole blood centrifuged at 460 × g for 8 min. PRGF F2 was incubated with 10% calcium chloride solution at 37℃ for 1 h to trigger platelet activation and growth factor release. Activated PRGF Fraction 2 was centrifuged at 3, 000 × g for 15 min, and the supernatant was then isolated. We examined the effects of soluble factors in PRGF on proliferation and mineralization in hMSC culture supplemented with PRGF. The proliferation of hMSC was increased in osteogenic induction medium(OIM)supplemented with PRGF. Alkaline phosphatase activity increased in hMSC by PRGF. Staining for alizarin red S and von Kossa was strong in hMSC supplemented with PRGF. These results suggest that PRGF is able to promote bone generation.

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