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A 105-day feed trial was conducted to evaluate the effect of probiotic feed supplement containing Saccharomyces cerevisiae on milk yield and its composition in Holstein Friesian cows. The trial was conducted in the region of Sidi Bouzid in the west of Tunisia. Effects of Saccharomyces cerevisiae have been investigated on eight Holstein Friesian cows randomly divided into two groups of four animals on the basis of age, body weight, average milk yield, and lactation number. The first group was supplemented with 2.5 g/cow/day of probiotic yeast Saccharomyces cerevisiae (2.5 1010 CFU/day) and the second group (control) was without the yeast. The study showed that supplementation with 2.5 g of yeast Saccharomyces cerevisiae per cow per day or 2.5 1010 CFU/day tended (P < 0.06) to increase milk production by 1.1 kg/cow. By cons, there was a significant increase of fat (P < 0.01; 52.8 and 46.9 g/cow/day) and protein (P < 0.05; 41.7 and 38.7 g/cow/day) content both for treated and control group, respectively. It is concluded that supplementation of Saccharomyces cerevisiae at 2.5 1010 CFU/day in the diet of dairy cows may have positive influence on milk fat and protei n yield (g/cow/day).

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This study aims to evaluate the nutritive value of Ajuga iva (A. iva) harvested from three distinct altitude regions in Tunisia (Dougga, Mograne, and Nabeul). The chemical composition, phenolic concentration, gas production, and in vitro dry matter (DM) digestibility were determined. The highest concentrations of neutral detergent fiber (NDF) and acid detergent fiber (ADF) were for A. iva cultivated in Nabeul. In contrast, the highest crude protein (CP) concentration was observed in that cultivated in Mograne, and the lowest (p < 0.01) CP concentration was noted in that cultivated in Dougga. Additionally, the cultivation regions affected the concentrations of free-radical scavenging activity, total flavonoids, and total polyphenols (p < 0.01). The highest free-radical scavenging activity was observed with A. iva cultivated in Dougga and Mograne. The highest (p < 0.05) gas production rate and lag time were observed in A. iva cultivated in Mograne and Nabeul regions. DM digestibility differed between regions and methods of determination. The highest (p < 0.01) DM degradability, determined by the method of Tilley and Terry and the method of Van Soest et al., was for A. iva cultivated in Mograne and Dougga, while the lowest (p < 0.01) value was recorded for that cultivated in the Nabeul region. Likewise, metabolizable energy (ME) and protein digestibility values were higher for A. iva collected from Mograne region than that collected from the other sampling areas. In conclusion, the nutritive value of A. iva differed between regions. Therefore, care should be taken when developing recommendations for using A. iva in an entire region. Season- and region-specific feeding strategies for feeding A. iva are recommended.

The present study was carried out to determine the phytochemical composition of Salvia officinalis flowers decoction extract (SOFDE) as well as its individual and/or synergistic actions with sulfasalazine against ethanol (EtOH)-induced peptic ulcer in Wistar rats.