Background Estimation of the post-mortem interval (PMI) is a crucial aspect in crime scene investigation. PMI is defined as the time between the moment of death and the moment of finding the dead body. A combination of methods for estimating the PMI in forensic casework is internationally mostly used. Supravital muscle reaction (SMR) is one of those methods. SMR is an idiomuscular contraction and can be provoked by mechanical stimulation. Objectives A field study was carried out with the aim to investigate whether a reflex hammer can be used as tool for triggering an idiomuscular contraction and, furthermore, to determine if a learning period has to be taken into account by a forensic physician for appropriate application of a reflex hammer to trigger SMR. Methods From January 2017 to January 2022, four forensic physicians used this SMR by mechanically stimulating the musculus brachioradialis and musculus biceps brachii. In total, 332 cases were included with a PMI less than 24 h. The cases were divided in chronological clusters of 20 cases. The ratio of the number of positive SMR versus the total number stimulations per forensic physician was used as a measure of accuracy of a reflex hammer for triggering SMR. The distribution of the data was analyzed by comparing the clusters in chronological order to assess whether a learning curve applies. Results In 55.7%, a muscle reaction could be provoked by mechanical stimulation. Comparable outcome of SMR between the participating physicians was observed after 40 stimulations. Conclusion A reflex hammer is usable for provoking SMR. A learning period has to be taken in to account during the first forty cases per forensic physician.