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Biosynthesis of cmdiolipin in liver mitochondria SUMMARY ~phosphatidyl~ycerol (cardiolipin) biosynthesis from CDP-diglyceride and s~-~ycerol-3-p~osphate has been demonstrated in a preparation of mitochondria from rat liver in a reaction requiring Mg'+. The identity of the ~phosphatidyl~ycerol was established by thin-layer chromato~aphy and mild alkaline hydrolysis. Diphosphatidylglycerol formation from [3H~pho~hatidyl~y&er~l was shown to occur only in the presence of ~P~~~y~eri~. The rate of ~phosp~tid~l~y~r~ formation appears to be quite slow in n~tochon~ia and may explain why it was not previously observed. Evidence is presented which eliminates the possibility of a bacterial contribution to the bio~nthetic process observed. Stanacev et al. * showed that cell-free preparations from Escherichia cdi could synthesize ~phosphatidyl~ycerol (car~olip~) in the presence of smglycerol3phosphate, CDPdiglyceride, Mga*, and Triton X-100. They presented good evidence for the following reaction sequence:

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A simple method is described for the preparation and use of stripping film of high quality for electron microscopic applications of autoradiography. The advantages of the method are: (a) uniform monolayers of emulsion are easily prepared with an inexpensive mechanical device and applied to thin sections on conventional grids;

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