In fluoroquinolone antibiotic group ciprofloxacin antibiotic is used for the treatment of human and animal infection because of their wide antibacterial spectrum. The rampant and extralable use of these antibiotics could lead to contamination of environment. This study describes a simple salting-out liquid-liquid extraction technique for simultaneously detection of ciprofloxacin residue in 13drinking water samples from two hospitals (Alacademy & Eltamouz). The high-performance liquid chromatographic ultra violet visible detection (HPLC-UV) containing C18 X-bridge analytical column was used for the determination of this antibiotic in water samples. The range of concentration of CIP in samples was between 3-654 µg/ ml. The developed method has linear range of 50-500 µg/ml with the linearity coefficient of 0.9989. The limit of detection and limit of quantification of the method for ciprofloxacin was 61.2 and 185.5 μg/ml respectively. The recoveries for the studied antibiotic ranged from 22.9-151.6% with relative standard deviations between 0.62 and 1.19%. The method has advantages of simplicity, easy operation and consumption of low volume of the less hazardous organic solvent.