African Animal Trypanosomosis (AAT) is a debilitating disease that hinders livestock productivity in Nigeria and sub-Saharan Africa. Numerous strategies have been developed over time to fight this devastating disease, which are emphasized mostly on containing the spread of its causative agent and principal vector. However, very little has been done to include livestock marketers in decision making, planning and implementation of control programs. Therefore, this study was carried out to fill that void, by evaluating the knowledge of this group of people on Tsetsefly and Trypanosomosis in Wudil Cattle Market. Questionnaires were developed to collect relevant information, and were administered through ‘Standard Focus Group Discussions’. The results revealed that tsetse fly was known by all respondents (100%), who significantly reported that they were most commonly found in the forests (95%), during the wet season (85%). Respondents also reported that these flies prefer to bite animals (71.25%). Similarly, a majority of respondents (97.5%) reported to being cognizant of trypanosomosis disease, while also stating that it had infected their animals at some point in time. Respondents believed infection was most prevalent during the wet season (60%) than the dry season (40%). Respondents had mixed views when it came to perceived causes of the disease, as some associated it with bite from flies (53.75%), while others linked it to transhumance (38.75%). In terms of signs and symptoms, 80% of respondents were able to identify with at least four symptoms of the disease. In essence, this study further intensifies the need to engage livestock marketers in tsetse and trypanosomosis control programs, in addition to emphasizing the need to create awareness campaigns that can further limit the spread of the disease and ensure vector control.