Lamotrigine. (LMT) is a triazine drug has an antiepileptic effect but with low water solubility, dissolution rate and thus therapeutic effect. Spanlastics are nano-vesicular carriers’ act as site-specific drug delivery system. Intranasal route could direct the drug from nose to brain and provide a faster and more specific therapeutic effect. Therefore, this study aimed to upload lamotrigine onto nano-vesicles using spanlastic nasal insert delivery for effective epilepsy treatment via overcoming lamotrigine’s low solubility and improving its bioavailability. Lamtrigine-loaded nano-spanlastic vesicles were prepared by ethanol injection method. To study different formulation factor’s effect on formulations characters; particle size (PS), Zeta potential (ZP), polydispersity index (PDI), entrapment efficiency percentage (EE%) and LMT released amount after 6 h (Q6h); 2^1 and 3^1 full factorial designs were employed. Optimized formula was loaded in lyophilized nasal inserts formulation which were characterized for LMT release and mucoadhesion. Pharmacokinetics studies in plasma and brain were performed on rats to investigate drug targeting efficiency. The optimal nano-spanlastic formulation (F4; containing equal Span 60 amount (100 mg) and edge activator; Tween 80) exhibited nano PS (174.2 nm), high EE% (92.75%), and Q6h > 80%. The prepared nasal inserts (S4) containing 100 mg HPMC has a higher mucoadhesive force (9319.5 dyne/cm 2 ) and dissolution rate (> 80% within 10 min) for rapid in vivo bio-distribution. In vivo studies showed considerable improvement brain and plasma’s rate and extent absorption after intranasal administration indicating a high brain targeting efficiency. The results achieved indicate that nano-spanlastic nasal-inserts offer a promising LMT brain targeting in order to maximize its antiepileptic effect.

Background: Wearing a facemask is one of the precautionary measures for preventing coronavirus disease spread, which causes many facial skin adverse effects. Vitamin-E (TPGS) and olive oil have an efficient effect on skin moistening. Therefore, in this study, face-masks embedded with NE; prepared using olive oil and TPGS, were developed to reduce the long-wearing face mask adverse effect. Methods: Box-Behnken design was used to develop NE formulations which were physically evaluated to select the most stable formulation. Fibroblast cell lines (3T3) were used to examine NE efficacy on dermal cell proliferation and in vitro cytotoxicity possibilities. Face-masks were also examined for 6 hr wearing dermal compatibility on healthy participants. Results: The formulated NE didn't significantly affect cell proliferation nor had a toxic effect on dermal cells (p<0.05). More than 60% of the respondents were satisfied with masks containing NE. Conclusion:In particular, a face mask embedded with the preparaed selected NE formula could produce emollient feelings during application without retarding the breathing or causing any irritant sensation.