The aim of this study was to investigate the effect of exercise with High Intensity Interval Training (HIIT) and Moderate Intensity Training (MIT) on inflammatory profile of proinflammatory cytokines TNF-α and IL-6 on the subject of obesity.This research is used 39 wistar rats of male-sex obesity. Intervention training is given for 6 weeks with frequency 4 times per week, HIIT intensity (90-100% of base line capability), MIT (60-80% of base line capability) TNF-α and IL-6 levels are obtained from blood testing with using ELISA test. Data analysis using Anova test.The results of this study showed that there were differences in levels of TNF-α HIIT with control (P = 0.003), MIT with control (P = 0.001) and no difference between HIIT group and MIT (P = 0.945). There was a difference of IL-6 levels between HIIT and control (P = 0.000), HIIT with MIT (P = 0.002), no difference in MIT group with control (P = 0.747). Exercise with the method of HIIT and MIT for 6 weeks with a frequency of 4 times per week can not create levels of TNF-α on the subject of obesity are lower as compared with sedentary groups, even the levels of TNF-α group of HIIT and MIT were significantly higher compared with the sedentary group. As for the levels of IL-6, HIIT is more effective in influencing the low IL-6 compared to MIT.