BACKGROUND: The impact of Coronavirus Disease-19 on peoples’ life has become evident. Recently, sport activities are important in improving individual health and their well-being. The aim of this study is to investigate the effect of using mobile phone fitness applications on some characteristics of physical fitness and psychological well-being of female university students. MATERIALS AND METHODS: A descriptive study design was implemented using a survey which was adapted to collect data from a sample of 50 female participants within the age of 18 to 19 years. As for the results, the participants indicated that they used physical fitness application by smart phone regularly during COVID-19. RESULTS: The results also illustrate that there was a significant difference on the study variables based on the study groups and test time. The study results extend literature concerning sport activities participation and promoting positive well-being during COVID-19 pandemic. CONCLUSION: The study indicated a significant difference of the application on physical fitness and psychological well-being among the participants. Based on the results, the study recommended to organize several sport activities among students and encourage them to use smart phones for positive purposes. Finally, practical implications are outlined.