Background: Permanent hearing loss is one of the most common congenital disorders, with an estimated incidence of one to three per thousand live births far exceeding the combined incidence of conditions for which newborns are routinely screened. Objective: The aim of this study was to estimate the incidence of hearing loss in newborn at Qena University Hospital, for early diagnosis, early intervention and good prognosis. Patients and Methods: This cross-sectional study was conducted at Qena University Hospital and 100 neonates were examined. The study was conducted in a duration of 6 months. Results: There was statistically significant relationship between the presence of risk factors such as prematurity, hyperbilirubinemia, hydrocephalus, respiratory distress syndrome, and amplitude of frequencies in both ears, there was no correlation between amplitudes of frequencies in both ears and age, heart rate, respiratory rate, incubation period or temperature. Conclusion: It could be concluded that otoacoustic Emission (OAE) is a reliable test for newborn hearing screening. Although all the 200 ears passed the screening test at Qena University Hospital, this does not guarantee a low prevalence of hearing loss but due to the limited number of cases and short duration of the study.