Hydrophobic charge induction chromatography (HCIC) has been proven to be an efficient technique for antibody purification. Several HCIC adsorbents were prepared with macroporous cellulose−tungsten carbide composite beads (Cell-TuC) as the matrix. First, the cellulose beads were activated by allyl bromide (AB) or divinyl sulfone (DVS), and then they were coupled with three types of mercaptoheterocyclic groups4-mercapto-ethyl-pyridine hydrochloride (MEP), 2-mercapto-1-methyl-imidazole (MMI), and 2-mercapto-benzimidazole (MBI)as the HCIC ligands. Four types of HCIC adsorbents were obtained, labeled Cell-TuC-AB-MEP, Cell-TuC-DVS-MEP, Cell-TuC-DVS-MMI, and Cell-TuC-DVS-MBI. The activation and coupling conditions were optimized for high ligand density. The isotherm adsorption of immunoglobulin of egg yolk (IgY) on four HCIC adsorbents were investigated. High adsorption capacities of IgY could be obtained for all four adsorbents at pH 7, and low adsorption of IgY at pH 4 and of bovine serum albumin (BSA) at pH 7 was observed, which indicates that the HCIC adsorbents prepared have a potential application for antibody purification.

In the current study, a GRAS (Generally Recognized As Safe) strain of Bacillus amyloliquefaciens producing 2,3-butanediol (2,3-BD) designated as B10-127 was isolated in our lab. The strain B10-127 produced 2,3-BD effectively under the condition of 20% glucose (quality concentration), showed a high-glucose tolerance. The effects of initial glucose concentration, temperature, pH and agitation on 2,3-BD production were investigated in this work and the proper parameters were identified. Accordingly, the fed-batch culture of B10-127 in larger scales (5 l) showed a remarkable 2,3-BD producing potency. The maximum 2,3-BD concentration reached 92.3 g/l at 96 h with a 2,3-BD productivity of 0.96 g/l h. To our knowledge, the results were new records on 2,3-BD fermentation by Bacillus, which shown an excellent candidate for the microbial fermentation of 2,3-BD on an industrial scale.

Acetoin (3-hydroxy-2-butanone), a very popular food spice is now used in many industries (pharmaceuticals, chemicals, paint, etc.). In this study, an acetoin high producing strain, numbered as JNA-310, was newly isolated and identified as Bacillus subtilis which is safe on food industry, based on its physiological, biological tests and 16S rDNA sequence analysis. When glucose was used as carbon source in fermentation, the fermentation characterizations of this strain were analyzed, and a new phenomenon of reverse transforming 2,3-butanediol which was synthesized from glucose in the fermentation broth to acetoin was detected. Before 96 h, glucose which was mainly transformed to 2,3-butanediol and acetoin was totally consumed, and the yield of the two products were 41.7 and 21.0 g/l respectively. Acetoin was only a by product in the fermentation broth at prophase of fermentation. At the end of fermentation, the yield of acetoin was greatly improved and the yield of 2,3-butanediol was declined and the yield of them were about 42.2 and 15.8 g/ l, respectively. The results indicated that 2,3-butanediol was reversely transformed to acetoin.