Senescence refers to a cellular state featuring a stable cell-cycle arrest triggered in response to stress. This response also involves other distinct morphological and intracellular changes including alterations in gene expression and epigenetic modifications, elevated macromolecular damage, metabolism deregulation and a complex pro-inflammatory secretory phenotype. The initial demonstration of oncogene-induced senescence in vitro established senescence as an important tumour-suppressive mechanism, in addition to apoptosis. Senescence not only halts the proliferation of premalignant cells but also facilitates the clearance of affected cells through immunosurveillance. Failure to clear senescent cells owing to deficient immunosurveillance may, however, lead to a state of chronic inflammation that nurtures a pro-tumorigenic microenvironment favouring cancer initiation, migration and metastasis. In addition, senescence is a response to post-therapy genotoxic stress. Therefore, tracking the emergence of senescent cells becomes pivotal to detect potential pro-tumorigenic events. Current protocols for the in vivo detection of senescence require the analysis of fixed or deep-frozen tissues, despite a significant clinical need for real-time Abbreviations 5-FU, 5-fluorouracil; AAH, atypical adenomatous hyperplasia, AIS adenocarcinoma in situ; ATM, ataxia-telangiectasia mutated; ATR, ATMand Rad3-related; B2M, b2-microglobulin; BAX, BCL2-associated protein X; BCL-2, B-cell lymphoma 2; BrdU, 5-bromo-2 0-deoxyuridine; C/ EBPb, CCAAT/enhancer-binding protein beta; CCF, cytoplasmic chromatin fragment; CDK, cyclin-dependent kinase; cfDNA, cell-free DNA; cGAS-STING, cyclic GMP-AMP synthase linked to stimulator of interferon genes; CHK, checkpoint kinase; CIS

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We developed a facile method for fabricating large-area, two-dimensional (2D), organic, highly crystalline films and extended it to organic thin-film transistor arrays. Tilted spinning provided oriented flow at the three-phase contact line, and a 2D crystalline film that consisted of layer-by-layer stacked 2,7-diocty[1]­benzothieno­[3,2-b]­benzothiophene (C8-BTBT) was obtained facilely for organic thin-film transistors (OTFTs). The extracted field-effect mobility is 4.6 cm2 V–1 s–1, but with nonideal features. By applying this method to microdroplet arrays, an oriented crystal was fabricated, and the channel region for OTFTs was covered by adjusting the spinning speed. By tuning the tilt angle (θ) of the revolving substrate, we fabricated high-performance OTFT arrays with average and maximum mobilities of 7.5 and 10.1 cm2 V–1 s–1, respectively, which exhibited high reliability factors of over 90% and were close to that of ideal transistors. These results suggest that high-quality crystalline films can be obtained via a facile tilted-spinning method.

In this work, we report an active respiration monitoring sensor based on a piezoelectric-transducer-gated thin-film transistor (PTGTFT) aiming to measure respiration-induced dynamic force in real time with high sensitivity and robustness. It differs from passive piezoelectric sensors in that the piezoelectric transducer signal is rectified and amplified by the PTGTFT. Thus, a detailed and easy-to-analyze respiration rhythm waveform can be collected with a sufficient time resolution. The respiration rate, three phases of respiration cycle, as well as phase patterns can be further extracted for prognosis and caution of potential apnea and other respiratory abnormalities, making the PTGTFT a great promise for application in long-term real-time respiration monitoring.