Aims: To identify a new fungal strain, Hypocrea sp. F000527 producing a trichothecene metabolite, harzianum A, and to evaluate its cytotoxicity to tumour cell lines. Methods and Results: A fungal strain, F000527, with cytotoxic activity was identified as a new Hypocrea strain based on morphological characteristics and internal transcribed spacers rDNA sequence data. Harzianum A was isolated from wheat bran culture by 50% acetone extraction, silica gel chromatography, Sephadex LH-20 chromatography and HPLC. The chemical structures were determined by ESI-or HRFAB-MS and 1 H and 13 C-NMR analyses. Harzianum A showed cytotoxicity to HT1080 and HeLa cell lines with IC 50 value of 0AE65 and 5AE07 lg ml )1 respectively.Conclusions: Harzianum A with a chemical formula of C 23 H 28 O 6 was isolated from a new Hypocrea strain and showed moderate to strong cytotoxicity to human cancer cell lines. Significance and Impact of the Study: This is the first report of the production of cytotoxic harzianum A by a new Hypocrea strain.

In this study, the complete mitochondrial genome of Laudakia wui, has been firstly determined by shotgun sequencing. The overall length of mitogenome is 16,455 bp and contains 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and 2 control regions. Majority of the genes (13 protein-coding genes, 2 rRNA and 14 tRNA) were distributed on the H-strand, in addition to the two ND6 genes and eight tRNA genes, which were encoded on the L-strand. The phylogenetic tree was built by L. wui and 13 other related species. The DNA data presented here will be useful to study the evolutionary relationships and genetic diversity of L. wui.