Introduction:Leachate is a noticeable pollution problem because it contains a considerable amount of persistent organic pollutants (POPs). If leachate isn't treated thoroughly, its leak will negatively affect the environment. Therefore, appropriate treatment technologies are required to remove them. Bio-Electro-Fenton (BEF) is a new method using microorganisms such as electrolytes to convert chemical energy into electricity to help create H 2 O 2 support advanced oxidation process (AOPs). Realizing the potentials that BEF brings, this study applies BEF to assess the effectiveness of leachate treatment at Da Phuoc landfill (operation period > 12 years), Ho Chi Minh City, which to save costs and energy for Fenton process. Methods: The BEF pilot scale model (30 x 10 x 10 cm) is divided by a proton exchange membrane (PEM) (Nafion ® 112) into two chambers (anode and cathode). Cathode chamber used a graphite electrode, the anode chamber used a carbon fabric electrode. The experiments aimed to determine the optimal conditions of parameters affecting the BEF system by determining the efficiency of COD removal and BOD 5 /COD ratio in leachate. Results: At optimal conditions of the model including pH 3, [Fe 2+ ] = 4g/L, current intensity = 1A, reaction time 60 minutes and airflow = 12 L/min, as a result COD was reduced by 68.2 ± 1.04 % from 4950 ±14 mgO 2 /L to 1574.1 ± 51.4 mgO 2 /L, the ratio of BOD 5 /COD = 0.1 Conclusion: The study result showed thatBio-electro-Fenton process is effective for wastewater with high concentrations of pollutant and difficult to treat as leachate suggesting that the appropriate method for pre-treatment processes support the thorough elimination of pollutants.