The objectives of this research was to determine the chemical composition of roscoe extract from methanol and evaluation of antibacterial activity. The phytochemical compound screened by GC-MS method. Forty eight bioactive phytochemical compounds were identified in the methanolic extract of Zingiber officinale. The identification of phytochemical compounds is based on the peak area, retention time molecular weight, molecular formula, MS Fragment-ions and pharmacological actions. GC-MS analysis of Zingiber officinale revealed the existence of the Octanal, 2-Naphthale namine, 1,2,4a,5,6,7,8, EndoBorneol, Decanal,1,[2][3][4][5][6][7][8][9][10][11][12][13][14][15] Propanal, Benzeneacetic acid ,2,3, 3´,15,16,21,22,(3ß,15á,16á,21ß,4,6a,7,8,9,10,10a,8,1, Naphthalene, decahydro-1-pentadecyl-, 13-Docosenamide,(Z)-, 9,10-Secocholesta-5,7,10(19)-triene-3,24,25-triol, (3ß,5Z,7E)-, n-(2,4-Dinitrophenyl)-N´-13-(2,6,6-trimethyl-cyclohex-1-enyl)propylider, n-(2,4-Dinitrophenyl)-N´-13-(2,6,6-trimethyl-cyclohex-1-enyl)propylider, Ingol 12-acetate, 2,2,8,12,7,11, Piperine,8,12,4,

Background: Hashimoto's Thyroiditis a common disease that affects the thyroid gland. Recent studies on Hashimoto's Thyroiditis indicated the ability of miRNA to silence messenger RNA and regulate post-transcriptional processes. Objective: The aim of the current study was to find the relationship between microRNA 125a-5p and the production of antibodies thyroid peroxidase (Anti-TPO). Patients and methods: The present study enrolled 125 patients, whom 25 were males and 100 females; the ratio of males to females was 1:4. Blood samples were collected from the participants from the Specialized Center for Endocrinology and Diabetes, Rusafa, Baghdad (Iraqi Ministry of Health). Hashimoto's patients were diagnosed using clinical methods, ultrasound, and serological testing. Also, anti-TPO were detected using the ELASI technique. Quantitative reverse transcription-polymerase chain reaction (RT-qPCR) was used to detect microRNA125a-5p levels in 40 samples from patients with Hashimoto's disease and compare them with 10 healthy individuals. Results: There were high levels of Anti-TPO in patients with Hashimoto's disease compared with the healthy individuals, with a statistically significant difference (P ≤0.01). The study also showed a decrease in the levels of miRNA 125a-5p in patients with Hashimoto's disease compared with the healthy individuals, with a statistically significant difference (P ≤0.01). Conclusion:The study showed a decreased miRNA-125a-5p expression that was found to be inversely associated with the low level for gene expect. This could be related to many factors as patients might be taking medication, population differentiation, races, miRNA, and drug interaction.

The prospected of this study was to determine the value of tumor-infiltrating macrophage cells with CD68+ a lysosomal associated membrane protein (LAMP) so as CD45+ which play important roles in cell recognition as a membrane glycoprotein. This clinical study used immune based biomarkers technique to prognosticate and evaluate for the entire effective activity of breast cancer (BC) disease in Iraqi women. The study concluded (34) female conflicted with BC in different stages of carcinoma, range aged (18-80 yrs) compared with (10) healthy individual. However CD68 and CD45+ markers were assessed by Immunohistochemical (IHC) staining of permanent tissue. The results of this study showed there were a significant differences of these markers compared with healthy control represented by highly significant leukocytes of CD45+ (P< 0.01) in absolutely of (+ve of 34/34) 100% for all patients with aggregates of tumors and high significant (P< 0.05) of tumorinfiltrating macrophage CD68+ 85.29 % (+ve 29 out of 34). The tested groups compared with the same healthy slide cleared with high percent in age (<40-80) for both clusters. The study concluded that there was a correlation between BC and tumor-infiltrating macrophage cells with CD68+ and CD45+ leukocyte common antigen as predicted markers detection. The collection of these observations suggest that CD68+ and CD45+ have an activation role so as differentiation of these clusters differentiation in leukocytes in breast cancer.