No abstract

Lettuce (Lactuca sativa) is a major crop and a member of the large, highly successful Compositae family of flowering plants. Here we present a reference assembly for the species and family. This was generated using whole-genome shotgun Illumina reads plus in vitro proximity ligation data to create large superscaffolds; it was validated genetically and superscaffolds were oriented in genetic bins ordered along nine chromosomal pseudomolecules. We identify several genomic features that may have contributed to the success of the family, including genes encoding Cycloidea-like transcription factors, kinases, enzymes involved in rubber biosynthesis and disease resistance proteins that are expanded in the genome. We characterize 21 novel microRNAs, one of which may trigger phasiRNAs from numerous kinase transcripts. We provide evidence for a whole-genome triplication event specific but basal to the Compositae. We detect 26% of the genome in triplicated regions containing 30% of all genes that are enriched for regulatory sequences and depleted for genes involved in defence.

Modifications of plant immune complexes by secreted pathogen effectors can trigger strong immune responses mediated by the action of nucleotide binding-leucine-rich repeat immune receptors. Although some strains of the pathogen Pseudomonas syringae harbor effectors that individually can trigger immunity, the plant’s response may be suppressed by other virulence factors. This work reveals a robust strategy for immune suppression mediated by HopZ3, an effector in the YopJ family of acetyltransferases. The suppressing HopZ3 effector binds to and can acetylate multiple members of the RPM1 immune complex, as well as two P. syringae effectors that together activate the RPM1 complex. These acetylations modify serine, threonine, lysine, and/or histidine residues in the targets. Through HopZ3-mediated acetylation, it is possible that the whole effector-immune complex is inactivated, leading to increased growth of the pathogen.

Soft robotics has yielded numerous examples of soft grippers that utilize compliance to achieve impressive grasping performances with great simplicity, adaptability, and robustness. Designing soft grippers with substantial grasping strength while remaining compliant and gentle is one of the most important challenges in this field. In this paper, we present a lightweight , vacuum-driven soft robotic gripper made of an origami "magic-ball" and a flexible thin membrane. We also describe the design and fabrication method to rapidly manufacture the gripper with different combinations of lowcost materials for diverse applications. Grasping experiments demonstrate that our gripper can lift a large variety of objects, including delicate foods, heavy bottles, and other miscellaneous items. The grasp force on 3D-printed objects is also characterized through mechanical load tests. The results reveal that our soft gripper can produce significant grasp force on various shapes using negative pneumatic pressure (vacuum). This new gripper holds the potential for many practical applications that require safe, strong, and simple grasping.