No abstract

No abstract

A serodiagnostic assay system to detect human anisakiasis is described. This assay uses a microenzyme-linked immunosorbent assay with monoclonal antibody An2 that recognizes the antigen specifically found in Anisakis simplex larvae. The data showed that sera from infected patients reacted strongly with the A. simplex larvae antigen that was immobilized with monoclonal antibody An2 on microplates. Patients' IgG, IgA, IgM, and IgE reacted strongly with An2-defined antigen at 4 or 5 weeks and at 1 or 7 days, respectively, after the onset of clinical symptoms of anisakiasis. However, sera from healthy persons did not react, even though they often ate raw fish. This indicates a high diagnostic specificity of this assay system for clinically manifested anisakiasis.

The serum of patients with parasitologically confirmed and one patient with a clinically presumptive case of anisakiasis were tested by the radioallergosorbent test (RAST) and counter-electrophoresis (CEP) for IgE and IgG antibodies, respectively, using antigens prepared from larval (L3) stage Anisakis simplex and larval (L2) stage Toxocara canis. All sera were RAST-positive to the A. simplex antigen and RAST-negative to the T. canis antigen. All sera were CEP-negative to both antigens. The presence of a specific IgE antibody suggests that the RAST could serve as a useful technique for the serodiagnosis of anisakiasis in man.

No abstract