Breast cancer is the most common cancer among women worldwide. This study was aimed to explain the experiences and knowledge of women in Jiroft city in iran regarding breast self-examination. This study was conducted with a combined approach in the research. After analyzing the quantitative data from the questionnaire, the perspectives of women on breast selfexamination were examined through interviews. The age range of the subjects was 20 years to 51 years, with a mean and standard deviation of (29.5±6.43). In the knowledge section, 95.8 percent failed to answer any of the questions, and 96% of subjects in this section emphasized that no educational program is provided by the health care team to perform breast self-examination. There was a significant relationship between knowledge and practice of breast self-examination (p=0.001). The following themes emerged in the results of a qualitative approach: 1) the feeling the need for breast self-examination; 2) attempt to play a role in the family; 3) lack of training programs on breast self-examination. Women's knowledge was found to be inadequate in relation to breast cancer and screening. Interventions to increase knowledge so as to achieve an adequate performance of breast self-examination, along with self-care and training programs, are tangibly needed on request.