No abstract

This work was involved DNA extraction from cultured Brachionus calyciflorus subjected to PCR .The PCR reaction was performed with Brachionus calyciflorus which having identical sequences of 18S rDNA and recorded as a global isolates of Brachionus calyciflorus in the (NCBI of USA, ENA of Europe and DDBJ of Japan) with Genbank ID: KM191796.1.

In this survey, six species of Brachionus genus are indexed as new record in the south Iraqi water resource at the first time in Iraq depending on the sequence of 18S rRNA gene,  these species  were registered as a new  record in Iraq and the other two species which are  HH1 and HH2 were registered in the National Center for Biotechnology Information (NCBI), The European  Nucleotide  Archive (ENA) and DNA Data Bank of Japan (DDBJ) sequentially.