The present work explored the preventive potential of ethanolic extract of Olea europaea L. (EEOE) leaves against CCl4-induced liver injury in rats. The fingerprint chromatogram of EEOE was determined by HPLC-MS analysis. The antioxidative potential of EEOE was determined by adopting three approved in vitro methods. The EEOE was orally given at a dose of 400 mg/kg, once a day, for 15 days continuously, succeeded by intraperitoneal (i.p.) injection of CCl4 (0.2%). The hepatoprotective potential was evaluated by estimating biochemical parameters including alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total bilirubin, total cholesterol, and triglycerides in the bloodstream. In vivo, the antioxidant ability against CCl4-induced liver injury in rats was assessed by estimating the levels of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), total reduced glutathione, and lipid peroxidation in the liver tissues. Further, histological analyses were performed to evaluate the degree of hepatic damage. Nine compounds were identified in the EEOE, principally oleuropein, luteolin-7-O-glucoside, and apigenin. The EEOE exhibited strong radical scavenging activity in DPPH assay. The EEOE significantly decreased the augmentation of serum cholesterol, TG, ALT, AST, ALP, and total bilirubin contents. It also restored hepatic SOD, CAT, GSH-Px activities, and glutathione (GSH) amount, and lowered lipid peroxidation amount comparable to the standard silymarin. The biochemical data were complemented with histological conclusions. These findings are indicative of the protective potential of the EEOE against CCl4-induced hepatotoxicity, which is possibly related to the potent antioxidative capacity of its phenolic compounds.

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This study was performed to evaluate the wound healing potential and antioxidant effect of methanol extract of Zizyphus lotus leaves (MZL), and to identify its phenolic profile by HPLC‐PDA‐ESI‐MS. Wound healing activity was assessed in vivo using excision model. Biochemical and histopathological analyses were conducted. In vitro antioxidant potential of MZL was tested using 2,2‐diphenyl‐1‐picrylhydrazyl and β‐carotene bleaching methods. HPLC‐PDA‐ESI‐MS was used for the phenolic profiling. Animals treated with MZL ointment showed a significant reduction in epithelialization time and high contraction rate compared to controls. Proteins, hydroxyproline, and hexosamines contents of ointment‐treated animals were higher than controls. Marked cross‐linking of collagen fibers and lack of inflammatory cells were observed in the granulation tissue of the ointment‐treated animals. Chemical profiling of MZL revealed that quercetin‐3‐O‐rutinoside and catechin are the major constituents. Phenolic compounds of the methanol extract of Zizyphus lotus leaves may be engaged in the acceleration of the healing process activity. Practical application In Mediterranean region, like Algeria, Zizyphus lotus is used in nutrition, health, and cosmetics. Several parts of this plant are employed to treat urinary troubles, diabetes, skin infections, fever, diarrhea, insomnia, bronchitis, and hypoglycemia. There is no scientific paper investigating the phenolic composition of Z. lotus leaves using a mass spectrometry coupled high‐performance liquid chromatography, as well as examining their wound healing potential. Results from this study revealed the presence of many flavonoids exhibiting important antioxidant and wound healing potentials through specific mechanisms. Z. lotus leaves are a source of pharmacologically active compounds with benefits for human nutrition, health‐promoting, and disease preventing.