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In this study, poly(vinyl chloride) (PVC) nanocomposite membranes with maghemite copper oxide nanocomposite (PMC) were developed in order to enhancethe PVC membrane performance and its physicochemical properties for the separation of oil-in-water emulsions. The maghemite copper oxide nanocomposite was synthesized by co-precipitation method. The nanocomposite-based membrane was fabricated by casting the nanocomposite onto a PVC support. By using the co-precipitation process, maghemite copper oxide nanocomposite was created. Various techniques such as Transmission electron microscope (TEM), Scanning electron microscope (SEM), X-ray diffraction (XRD), and Fourier transform infrared (FT-IR), have been used to characterize membranes. Water flux, solute rejection, and anti-fouling properties of the membrane were also evaluated. The results demonstrated the nanocomposite's high degree of crystallinity and well-dispersed nanostructure. The resulting membrane was found to have a high oil-water separation efficiency of 98% and an ultra-flux of 0.102L/m2.h compared pure PVC membrane. It was found that the rejection efficiency remained stable over5 cycle times with excellent anti-fouling and cycling stability. The results showed that the maghemite copper oxide nanocomposite-based PVC membrane is a promising material for use in the separation of oil-in-water emulsions, which provide valuable information for the development of new membrane and more flexible, durable, and anti-fouling, making it ideal for use in the separation of oil-in-water emulsions.