In recent times, the modifiable lifestyle factors have been shown to affect the chance of conception in both infertile and general population. There is strong evidence that weight, smoking, alcohol consumption, caffeine intake, nutritional habits, physical activity, Stress, exposure to animals, pollution and Recreational drugs had an adverse impact on reproductive health. Aim: assess the perception of infertile couples regarding the effect of lifestyle factors on fertility; compare the lifestyle factors between fertile and infertile couples. Methods: A descriptive cross-sectional design was used to conduct this study. It was conducted at infertility outpatient clinic women's health Hospitals, Assiut University, Egypt. Sample: included 117 infertile couples. Structured interview questionnaire was used and included two parts: Socio-demographic data and questions to assess perception of infertile couples regarding lifestyle. Simple Lifestyle Indicator Questionnaire. Results: A highly statistically difference was found between infertile husbands and wives regarding perceived the smoking habits. No significant difference was found among infertile couples regarding drinking alcohol, over or underweight, stress, exposure to animals and environmental pollution, dietary habits, and influence of caffeine, exercises and taking recreational drugs. Conclusion: perceptions of the impact of lifestyle factors on fertility vary by sex, socioeconomic factors and treatment. Lifestyle factors significantly affected couples fertility, as BMI, exercises, nutritional status and stress with a statistical difference between groups. Recommendation: In-services educational program for infertile couples for maintaining lifestyle habits to improve their chance of spontaneous conception.