Hepatocellular carcinoma (HCC) is a prevalent human malignancy which its drug resistance is increasing world-wide. This project was designed to assess the anti-cancer effects of 4-bromo-2-(((5-chloro-2-hydroxyphenyl) imino) methyl) phenol ([IV(L)] complex) on the HepG2 cell line and also L929 cells, as normal cells. HepG2 and L929 cells were cultured in RPMI culture medium and the survival rates of the cells were determined after 24 and 48 h using MTT assay to find IC50 concentration of vanadium m, [IV(L)] complex. The early apoptosis and necrosis/late apoptosis were determined by means of annexin V/PI apoptosis detection kit. The results revealed that vanadium m, [IV(L)] complex induce early apoptosis higher in HepG2 cell line than L929 cells. The rates of necrosis/late apoptosis were also induced in HepG2 cells more than L929 cells. Based on the results, vanadium m, [IV(L)] complex might be considered as a safe new drug for treatment of HCC with low side effects on control liver cells.

Background and purpose: Cancer is the primary cause of death in the world. Vanadium (IV), a metal ion complex, has been proposed as an appropriate applicant for cancer treatment. In this study, the interaction of an oxidovanadium (IV) complex [VOL(bipy)] with salmon sperm DNA and bovine serum albumin (BSA) was investigated through experimental and computational approaches Using the results of this experimental study, the mechanism as well as the parameters related to the interaction of [VOL(bipy)] with DNA and BSA was determined. Material and methods: The kinetic DNA and BSA interaction with [VOL(bipy)] was determined using absorption titration and fluorescence quenching, respectively. Moreover, the possible interactions was calculated by molecular docking prediction with available software. Results: The binding constant (Kb) of the complex to DNA was calculated to be 2.34×104 M-1 , indicating a relatively strong interaction between the complex and DNA. It was found that the V(IV) complex interacted with DNA through groove binding mode followed by partial intercalation into the DNA helix. The Kb values obtained for [VOL(bipy)]-BSA interaction were in the range of 1.07×103 -5.82×104 M-1 . The V(IV) complex was found to prefer domain I binding pocket of BSA with the ΔGb value of -7.52 kcal/mol. Conclusion: Both experimental and computational studies confirm the interaction of the Vanadium complex with DNA and BSA. The moderate affinity of [VOL(bipy)] to BSA indicates that this protein is a good candidate for transferring the complex.

Introduction: Social acceptance is defined as the support by social network for patients via family, friends and colleagues that often reason the better management and prognosis. If the social acceptance is significantly low in patients with chronic diseases like tuberculosis, an appropriate plan can raise the level of knowledge and the culture of the community to enhance the level of patient and community's acceptance. The authors aim to evaluate social acceptance and related factors in these patients in Rafsanjan. Methods: This was a descriptive and cross-sectional study. It was conducted on 29 pulmonary TB (tuberculosis) patients who had referred to Rafsanjan city health center in Iran in 2014. They were selected by census method. The Marlou-Crown questionnaire was completed by patients, and the scores were calculated based on the questionnaire key using SPSS 16. T-test, Fisher and chi-square tests were used for evaluation, and the significance level was P-value <0.05. Results: Results showed that the frequency social acceptance among patients was moderate to high (62%) and moderate to low (38%). Fifteen (52%) patients were male and 14 (48%) were female whose age range was 8 to 90.  Among the variables of age, sex, education, place of residence, nationality and family history of patients, the relationship between age، nationality, positive family history of TB and social acceptance was significant (P-value <0.05). Conclusion: This study concludes that older patients, those with a family history of TB, and migrant patients have higher social acceptance (P-value <0.05).