In this article, a new observer design technique is proposed for the general affine class of fractional-order nonlinear multi-input/multi-output (MIMO) systems. This study is presented based on the differential mean value theorem. One significant characteristic of the suggested approach is that the nonlinear dynamic of observer error is converted into a linear parameter-varying system. Stability and convergence of observation error are shown using the Lyapunov direct method leading to feasibility and existence of a solution for some linear matrix inequalities. The performance and efficacy of the proposed method are evaluated through some illustrated simulations.