University is a period characterized by an absence of parental supervision and independence. Few research has been conducted in Morocco among this particular population, the aim of this study is to assess the prevalence to substance use among student, to develop knowledge of how and why substances are used and or abused.There were 1054 participants, divided into university student group (n=444) and non-student group (n=610) using a random sampling process for the university student group, and multistage stratified probability procedure for the other.The current study revealed that tobacco use was found the most prevalent substance used among university student representing 65,1%. While the prevalence of cannabis use was 29,1%, and psychotropic drugs was 2,5%.This study has demonstrated a high prevalence of substance use among young educated adults who should be role models for their community, fighting substance use rather than using it.