Background: The purpose of this study was to evaluate the effects of acute resistance training on changes in biochemical markers of myocardial injury (cTnT, cTnI, CK-MB) in non-athlete women. Methods: In this study, 18 healthy young women with a mean age (23.41 years) and body fat percent (21.62) participated voluntarily. Untrained women performed 7 resistance exercises at 70% 1RM in 3 sets and 10 repetitions. Rest interval between sets was 2 minutes. Blood samples were obtained before, immediately, 1, 24, and 48 hours after exercise. Statistical analyses were conducted using ANOVA with repeated measure (P ≤ 0.05). Results: The results revealed a significant difference in troponin I in pre-test immediately and 1 hour after exercise, but 24 hours and 48 hours after, it returned to baseline. There was also a significant difference in troponin T and CK-MB in pre-test immediately and 1 hour and 24 hours after exercise, but they returned to baseline after 48 hours. Conclusions: It can be concluded that resistance exercise may lead to an increase in cardiac troponin and CK iso-enzyme. However, it seems to be a temporary increment and do not cause cardiac damage.